ArticleNature structural & molecular biology2010
Direct observation of the myosin-Va power stroke and its reversal.
Article in Nature structural & molecular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed.
- Characterizing the concentration and load dependence of phosphate binding to rabbit fast skeletal actomyosin.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Motor domain phosphorylation increases nucleotide exchange and turns MYO6 into a faster and stronger motor.Nature communications · 2024Article
- Myosin-5 varies its step length to carry cargo straight along the irregular F-actin track.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Myosin-5 varies its steps along the irregular F-actin track.bioRxiv : the preprint server for biology · 2023Article
- A mutation in switch I alters the load-dependent kinetics of myosin Va.Nature communications · 2023Article
- Multistep orthophosphate release tunes actomyosin energy transduction.Nature communications · 2022Article
- Structural basis for tunable control of actin dynamics by myosin-15 in mechanosensory stereocilia.Science advances · 2022Article
- Recent insights into the relative timing of myosin's powerstroke and release of phosphate.Cytoskeleton (Hoboken, N.J.) · 2021Review
- A reverse stroke characterizes the force generation of cardiac myofilaments, leading to an understanding of heart function.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Mechanism of contraction rhythm homeostasis for hyperthermal sarcomeric oscillations of neonatal cardiomyocytes.Scientific reports · 2020Article
- Squeezing in a Meal: Myosin Functions in Phagocytosis.Trends in cell biology · 2020Review
- Article
- A modular DNA scaffold to study protein-protein interactions at single-molecule resolution.Nature nanotechnology · 2019Article
- Effect of myofibril passive elastic properties on the mechanical communication between motor proteins on adjacent sarcomeres.Scientific reports · 2019Article
- Step Sizes and Rate Constants of Single-headed Cytoplasmic Dynein Measured with Optical Tweezers.Scientific reports · 2018Article
- Dissecting myosin-5B mechanosensitivity and calcium regulation at the single molecule level.Nature communications · 2018Article
- Active Mechanics Reveal Molecular-Scale Force Kinetics in Living Oocytes.Biophysical journal · 2018Article
- Myosin V: Chemomechanical-coupling ratchet with load-induced mechanical slip.Scientific reports · 2017Article
- Kinematics of the lever arm swing in myosin VI.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Simulating the dynamics of the mechanochemical cycle of myosin-V.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Complex forms of cellular motility, including cell division, organelle trafficking or signal amplification in the auditory system, require strong coordination of the myosin motors involved. The most basic mechanism of coordination is via direct mechanical interactions of individual motor heads leading to modification of their mechanochemical cycles. Here we used an optical trap-based assay to investigate the reversibility of the force-generating conformational change (power stroke) of single myosin-Va motor heads. By applying load to the head shortly after binding to actin, we found that, at a certain load, the power stroke could be reversed, and the head fluctuated between an actin-bound pre- and a post-power stroke conformation. This load-dependent mechanical instability might be critical to coordinate the heads of processive, dimeric myosin-Va. Nonlinear response to load leading to coordination or oscillations amongst motors might be relevant for many cellular functions.
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Registered trials
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