ArticleNature cell biology2010
Electron tomography reveals unbranched networks of actin filaments in lamellipodia.
Article in Nature cell biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 90 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
90 citing papers in PubMed, 207 citations in OpenAlex.
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- Macrophages bend long fibres with flexural rigidity lower than 3 mN·nmParticle and fibre toxicology · 2026Article
- Cryo-electron tomography reveals OmpB is required for themicroPublication biology · 2026Article
- Cryo-ET of actin cytoskeleton and membrane structure in lamellipodia formation using optogenetics.iScience · 2025Article
- Handling Difficult Cryo-ET Samples: A Study with Primary Neurons from Drosophila melanogaster.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2023Article
- Mechanisms of actin disassembly and turnover.The Journal of cell biology · 2023Review
- At the Nexus between Cytoskeleton and Vacuole: How Plant Cytoskeletons Govern the Dynamics of Large Vacuoles.International journal of molecular sciences · 2023Review
- How torque on formins is relaxed strongly affects cellular swirling.Biophysical journal · 2022Article
- Leading edge maintenance in migrating cells is an emergent property of branched actin network growth.eLife · 2022Article
- 4polar-STORM polarized super-resolution imaging of actin filament organization in cells.Nature communications · 2022Article
- Moving through a changing world: Single cell migration in 2D vs. 3D.Frontiers in cell and developmental biology · 2022Review
- The WAVE complex associates with sites of saddle membrane curvature.The Journal of cell biology · 2021Article
- Filamin A Regulates Cardiovascular Remodeling.International journal of molecular sciences · 2021Review
- Numerical analysis of the impact of cytoskeletal actin filament density alterations onto the diffusive vesicle-mediated cell transport.PLoS computational biology · 2021Article
- Optogenetic manipulation of cellular communication using engineered myosin motors.Nature cell biology · 2021Article
- Predictive assembling model reveals the self-adaptive elastic properties of lamellipodial actin networks for cell migration.Communications biology · 2020Article
- Article
- Three-dimensional organization of the cytoskeleton: A cryo-electron tomography perspective.Protein science : a publication of the Protein Society · 2020Review
- Loss of Ena/VASP interferes with lamellipodium architecture, motility and integrin-dependent adhesion.eLife · 2020Article
30 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Eukaryotic cells can initiate movement using the forces exerted by polymerizing actin filaments to extend lamellipodial and filopodial protrusions. In the current model, actin filaments in lamellipodia are organized in a branched, dendritic network. We applied electron tomography to vitreously frozen 'live' cells, fixed cells and cytoskeletons, embedded in vitreous ice or in deep-negative stain. In lamellipodia from four cell types, including rapidly migrating fish keratocytes, we found that actin filaments are almost exclusively unbranched. The vast majority of apparent filament junctions proved to be overlapping filaments, rather than branched end-to-side junctions. Analysis of the tomograms revealed that actin filaments terminate at the membrane interface within a zone several hundred nanometres wide at the lamellipodium front, and yielded the first direct measurements of filament densities. Actin filament pairs were also identified as lamellipodium components and bundle precursors. These data provide a new structural basis for understanding actin-driven protrusion during cell migration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.