ArticleMolecular & cellular proteomics : MCP2010
Comprehensive characterization of methicillin-resistant Staphylococcus aureus subsp. aureus COL secretome by two-dimensional liquid chromatography and mass spectrometry.
Article in Molecular & cellular proteomics : MCP, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
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- Protein trafficking across the cell envelope of gram-positive bacteria.Journal of bacteriology · 2025Review
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- Recent Advances in Genomics-Based Approaches for the Development of Intracellular Bacterial Pathogen Vaccines.Pharmaceutics · 2022Review
- Characterization of the Secreted Acid Phosphatase SapS Reveals a Novel Virulence Factor ofInternational journal of molecular sciences · 2022Article
- The Major Autolysin Atl Regulates the Virulence of Staphylococcus aureus by Controlling the Sorting of LukAB.Infection and immunity · 2022Article
- Article
- Advanced strategies for development of vaccines against human bacterial pathogens.World journal of microbiology & biotechnology · 2021Review
- Identification of CD4Biotechnologia · 2021Article
- Peptide-based quorum sensing systems inLife science alliance · 2020Article
- AViruses · 2020Article
- Bacterial MgrB peptide activates chemoreceptor Fpr3 in mouse accessory olfactory system and drives avoidance behaviour.Nature communications · 2019Article
- Identification of N-terminal protein processing sites by chemical labeling mass spectrometry.Rapid communications in mass spectrometry : RCM · 2019Article
- Intact Staphylococcus Enterotoxin SEB from Culture Supernatant Detected by MALDI-TOF Mass Spectrometry.Toxins · 2019Article
- Septal secretion of protein A ineLife · 2018Article
- Article
- Aspartate tightens the anchoring of staphylococcal lipoproteins to the cytoplasmic membrane.MicrobiologyOpen · 2017Article
- Not just an antibiotic target: Exploring the role of type I signal peptidase in bacterial virulence.Bioorganic & medicinal chemistry · 2016Article
- SDS-PAGE and Gel IEF: Tool for Differentiation of Methicillin-Resistant and Methicillin-Sensitive Strains of Staphylococcus aureus.Current microbiology · 2016Article
- Identification of commonly expressed exoproteins and proteolytic cleavage events by proteomic mining of clinically relevant UK isolates ofMicrobial genomics · 2016Article
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2 authors.
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Abstract
Two-dimensional LC combined with whole protein and peptide mass spectrometry is used to characterize proteins secreted by methicillin-resistant Staphylococcus aureus COL. Protein identifications were accomplished via off-line protein fractionation followed by digestion and subsequent peptide analysis by reverse phase LC-ESI-LTQ-FT-MS/MS. Peptide MS/MS analysis identified 127 proteins comprising 59 secreted proteins, seven cell wall-anchored proteins, four lipoproteins, four membrane proteins, and 53 cytoplasmic proteins. The identified secreted proteins included various virulence factors of known functions (cytotoxins, enterotoxins, proteases, lipolytic enzymes, peptidoglycan hydrolases, etc.). Accurate whole protein mass measurement (+/-1.5 Da) of the secreted proteins combined with peptide analysis enabled identification of signal peptide cleavage sites and various post-translational modifications. In addition, new observations were possible using the present approach. Although signal peptide cleavage is highly specific, signal peptide processing can occur at more than one site. Surprisingly, cleaved signal peptides and their fragments can be observed in the extracellular medium. The prediction accuracies of several signal peptide prediction programs were also evaluated.
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