ArticleApplied and environmental microbiology2010
TDP-L-megosamine biosynthesis pathway elucidation and megalomicin a production in Escherichia coli.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- New Marine Actinobacteria Strain,Microorganisms · 2025Article
- Antibacterial activity and biosynthetic potential of Streptomyces sp. PBR19, isolated from forest rhizosphere soil of Assam.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Nematicidal glycosylated resorcylic acid lactones from the fungusFrontiers in microbiology · 2024Article
- OSMAC Method to Assess Impact of Culture Parameters on Metabolomic Diversity and Biological Activity of Marine-Derived Actinobacteria.Marine drugs · 2023Article
- New Insights on Biological Activities, Chemical Compositions, and Classifications of Marine Actinomycetes Antifouling Agents.Microorganisms · 2023Review
- Prioritization of Microorganisms Isolated from the Indian Ocean SpongeMicroorganisms · 2023Article
- Engineering actinomycetes for biosynthesis of macrolactone polyketides.Microbial cell factories · 2019Review
- Enzymatic Synthesis of the C-Glycosidic Moiety of Nogalamycin R.ACS chemical biology · 2018Article
- Glycogenomics as a mass spectrometry-guided genome-mining method for microbial glycosylated molecules.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Chemobiosynthesis of new antimalarial macrolides.Antimicrobial agents and chemotherapy · 2013Article
- Structure of the glycosyltransferase EryCIII in complex with its activating P450 homologue EryCII.Journal of molecular biology · 2012Article
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6 authors.
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Abstract
In vivo reconstitution of the TDP-l-megosamine pathway from the megalomicin gene cluster of Micromonospora megalomicea was accomplished by the heterologous expression of its biosynthetic genes in Escherichia coli. Mass spectrometric analysis of the TDP-sugar intermediates produced from operons containing different sets of genes showed that the production of TDP-l-megosamine from TDP-4-keto-6-deoxy-d-glucose requires only five biosynthetic steps, catalyzed by MegBVI, MegDII, MegDIII, MegDIV, and MegDV. Bioconversion studies demonstrated that the sugar transferase MegDI, along with the helper protein MegDVI, catalyzes the transfer of l-megosamine to either erythromycin C or erythromycin D, suggesting two possible routes for the production of megalomicin A. Analysis in vivo of the hydroxylation step by MegK indicated that erythromycin C is the intermediate of megalomicin A biosynthesis.
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