ArticleApplied and environmental microbiology2010
Widespread head-to-head hydrocarbon biosynthesis in bacteria and role of OleA.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 120 citations in OpenAlex.
- Directed evolution of hydrocarbon-producing enzymes.Biotechnology for biofuels and bioproducts · 2025Review
- Exploring 1-alkene biosynthesis in bacterial antagonists and Jeotgalicoccus sp. ATCC 8456.FEMS microbiology letters · 2025Article
- Biosynthesis pathways of expanding carbon chains for producing advanced biofuels.Biotechnology for biofuels and bioproducts · 2023Review
- Genomic insights into cryptic cycles of microbial hydrocarbon production and degradation in contiguous freshwater and marine microbiomes.Microbiome · 2023Article
- Recombinant Expression and Purification of Large Bacterial Multienzyme Assemblies for Biosynthetic Processes.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Microbial engineering to produce fatty alcohols and alkanes.Journal of industrial microbiology & biotechnology · 2021Review
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- Undecane production by cold-adapted bacteria from Antarctica.Extremophiles : life under extreme conditions · 2020Article
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- Repurposing Modular Polyketide Synthases and Non-ribosomal Peptide Synthetases for Novel Chemical Biosynthesis.Frontiers in molecular biosciences · 2020Review
- Distribution and diversity of olefins and olefin-biosynthesis genes in Gram-positive bacteria.Biotechnology for biofuels · 2020Article
- Linkage of Marine Bacterial Polyunsaturated Fatty Acid and Long-Chain Hydrocarbon Biosynthesis.Frontiers in microbiology · 2019Article
- Recent Advances in Enzymatic Complexity Generation: Cyclization Reactions.Biochemistry · 2018Review
- Variances in cellular sedimentation behavior as an effective enrichment method of hydrocarbon-overproducingBiotechnology for biofuels · 2018Article
- Impact of Branched-Chain Amino Acid Catabolism on Fatty Acid and Alkene Biosynthesis inFrontiers in microbiology · 2018Article
- OleA Glu117 is key to condensation of two fatty-acyl coenzyme A substrates in long-chain olefin biosynthesis.The Biochemical journal · 2017Article
- Biobased production of alkanes and alkenes through metabolic engineering of microorganisms.Journal of industrial microbiology & biotechnology · 2017Review
- Active Multienzyme Assemblies for Long-Chain Olefinic Hydrocarbon Biosynthesis.Journal of bacteriology · 2017Article
- β-Lactone Synthetase Found in the Olefin Biosynthesis Pathway.Biochemistry · 2017Article
- Microbial alkane production for jet fuel industry: motivation, state of the art and perspectives.Microbial biotechnology · 2017Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previous studies identified the oleABCD genes involved in head-to-head olefinic hydrocarbon biosynthesis. The present study more fully defined the OleABCD protein families within the thiolase, alpha/beta-hydrolase, AMP-dependent ligase/synthase, and short-chain dehydrogenase superfamilies, respectively. Only 0.1 to 1% of each superfamily represents likely Ole proteins. Sequence analysis based on structural alignments and gene context was used to identify highly likely ole genes. Selected microorganisms from the phyla Verucomicrobia, Planctomyces, Chloroflexi, Proteobacteria, and Actinobacteria were tested experimentally and shown to produce long-chain olefinic hydrocarbons. However, different species from the same genera sometimes lack the ole genes and fail to produce olefinic hydrocarbons. Overall, only 1.9% of 3,558 genomes analyzed showed clear evidence for containing ole genes. The type of olefins produced by different bacteria differed greatly with respect to the number of carbon-carbon double bonds. The greatest number of organisms surveyed biosynthesized a single long-chain olefin, 3,6,9,12,15,19,22,25,28-hentriacontanonaene, that contains nine double bonds. Xanthomonas campestris produced the greatest number of distinct olefin products, 15 compounds ranging in length from C(28) to C(31) and containing one to three double bonds. The type of long-chain product formed was shown to be dependent on the oleA gene in experiments with Shewanella oneidensis MR-1 ole gene deletion mutants containing native or heterologous oleA genes expressed in trans. A strain deleted in oleABCD and containing oleA in trans produced only ketones. Based on these observations, it was proposed that OleA catalyzes a nondecarboxylative thiolytic condensation of fatty acyl chains to generate a beta-ketoacyl intermediate that can decarboxylate spontaneously to generate ketones.
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