ArticleRNA (New York, N.Y.)2010
A versatile ribosomal protein promoter-based reporter system for selective assessment of RNA stability and post-transcriptional control.
Article in RNA (New York, N.Y.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 29 citations in OpenAlex.
- Deciphering the Contribution of TATA Box and 5'UTR to Defense Signaling in Rice Under Blast Infection.Biology · 2025Review
- Identification of immune signatures associated with both SARS-CoV-2 infection and lung transplantation.Cell reports. Medicine · 2025Article
- Post-transcriptional regulation of BIRC5/survivin expression and induction of apoptosis in breast cancer cells by tristetraprolin.RNA biology · 2024Article
- Beyond the exome: utility of long-read whole genome sequencing in exome-negative autosomal recessive diseases.Genome medicine · 2023Article
- Differential upregulation of AU-rich element-containing mRNAs in COVID-19.Human genomics · 2022Article
- Kinome inhibition reveals a role for polo-like kinase 1 in targeting post-transcriptional control in cancer.Molecular oncology · 2021Article
- Bi-phased regulation of the post-transcriptional inflammatory response by Tristetraprolin levels.RNA biology · 2019Article
- Post-Transcriptional Inflammatory Response to Intracellular Bacterial c-di-AMP.Frontiers in immunology · 2019Article
- Identification of a novel genetic locus underlying tremor and dystonia.Human genomics · 2017Article
- A novel mechanism for variable phenotypic expressivity in Mendelian diseases uncovered by an AU-rich element (ARE)-creating mutation.Genome biology · 2017Article
- Human Cytokinome Analysis for Interferon Response.Journal of virology · 2015Article
- High-throughput screening for chemical modulators of post-transcriptionally regulated genes.Journal of visualized experiments : JoVE · 2015Article
- Posttranscriptional control of the chemokine receptor CXCR4 expression in cancer cells.Carcinogenesis · 2014Article
- Sustained stabilization of Interleukin-8 mRNA in human macrophages.RNA biology · 2014Article
- miR-29a inhibition normalizes HuR over-expression and aberrant AU-rich mRNA stability in invasive cancer.The Journal of pathology · 2013Article
- Regulation of p21/CIP1/WAF-1 mediated cell-cycle arrest by RNase L and tristetraprolin, and involvement of AU-rich elements.Nucleic acids research · 2012Article
- UU/UA dinucleotide frequency reduction in coding regions results in increased mRNA stability and protein expression.Molecular therapy : the journal of the American Society of Gene Therapy · 2012Article
- A daphnane diterpenoid isolated from Wikstroemia polyantha induces an inflammatory response and modulates miRNA activity.PloS one · 2012Article
- Green fluorescent protein reporter system with transcriptional sequence heterogeneity for monitoring the interferon response.Journal of virology · 2011Article
- Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Assessment of post-transcriptional control relies on use of transcriptional inhibitors and is masked by copious and cryptic transcriptional induction. We screened several cellular promoters that are constitutively active yet noninducible to external stimuli. The ribosomal protein RPS30 promoter was chosen; its TATA signal and sp1 site location were optimized. The modified promoter (RPS30M) is selective to post-transcriptional effects of AU-rich elements (ARE) in the 3'UTR, while it is not transcriptionally responsive to a wide variety of agents including pro-inflammatory cytokines and RNA-binding proteins. Specific cis-acting elements can be appended to RPS30M by a cloning-free approach to allow coupled transcriptional/post-transcriptional assessment, as demonstrated with NF-kappaB and beta-catenin/wnt signaling experiments. Moreover, efficient tetracycline-regulated RPS30M was created for quantitative assessment of the half-lives of mRNAs containing AREs. The described approach provides enhanced versatility and suitability for selective post-transcriptional assessment with or without transcriptional induction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.