ArticleThe Annals of thoracic surgery2010
In vivo fluorometric assessment of cyclosporine on mitochondrial function during myocardial ischemia and reperfusion.
Article in The Annals of thoracic surgery, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
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Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it, 27 citations in OpenAlex.
- Cyclosporin variably and inconsistently reduces infarct size in experimental models of reperfused myocardial infarction: a systematic review and meta-analysis.British journal of pharmacology · 2012Pooled it
- Redox Equivalents and Mitochondrial Bioenergetics.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Urotensin-ⅡReceptor Antagonist SB-710411 Protects Rat Heart against Ischemia-Reperfusion Injury via RhoA/ROCK Pathway.PloS one · 2016Article
- Ageing-related changes in GABAergic inhibition in mouse auditory cortex, measured using in vitro flavoprotein autofluorescence imaging.The Journal of physiology · 2016Article
- Optical cryoimaging of mitochondrial redox state in bronchopulmonary-dysplasia injury models in mice lungs.Quantitative imaging in medicine and surgery · 2015Article
- Organ specific optical imaging of mitochondrial redox state in a rodent model of hereditary hemorrhagic telangiectasia-1.Journal of biophotonics · 2014Article
- Optical imaging of mitochondrial redox state in rodent models with 3-iodothyronamine.Experimental biology and medicine (Maywood, N.J.) · 2014Article
- Novel Flurometric Tool to Assess Mitochondrial Redox State of Isolated Perfused Rat Lungs after Exposure to Hyperoxia.IEEE journal of translational engineering in health and medicine · 2013Article
- Surface fluorescence studies of tissue mitochondrial redox state in isolated perfused rat lungs.Annals of biomedical engineering · 2013Article
- Optical imaging of mitochondrial redox state in rodent model of retinitis pigmentosa.Journal of biomedical optics · 2013Article
- Optical imaging of tissue mitochondrial redox state in intact rat lungs in two models of pulmonary oxidative stress.Journal of biomedical optics · 2012Article
- What yeast and cardiomyocytes share: ultradian oscillatory redox mechanisms of cellular coherence and survival.Integrative biology : quantitative biosciences from nano to macro · 2012Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundCyclosporine A (CsA) limits myocardial reperfusion injury and preserves mitochondrial integrity, but its influence on mitochondrial function has not been described in vivo. Auto-fluorescence of mitochondrial nicotinamide adenine dinucleotide and flavin adenine dinucleotide correlate with mitochondrial dysfunction. We hypothesized that CsA limits mitochondrial dysfunction and that fluorometry can quantify this influence.
methodsSeventeen rabbits were studied: untreated (UnT, n = 7), CsA preinfarction (CsAp, n = 6), and CsA on reperfusion (CsAr, n = 4). Animals underwent 30 minutes of myocardial ischemia and 3 hours reperfusion. Infarct size was determined by staining. Nicotinamide adenine dinucleotide and flavin adenine dinucleotide fluorescence was continually measured in the risk area. The redox ratio was calculated [flavin adenine dinucleotide(f)/(flavin adenine dinucleotide(f) + nicotinamide adenine dinucleotide(f))]. Electron microscopy evaluated mitochondria morphology.
resultsThe infarct size by group was 39.1% +/- 1.7% in CsAp, 39.1% +/- 1.7% in CsAr, and 53.4% +/- 1.9% in UnT (p < 0.001). During ischemia, the CsAp group demonstrated less hypoxic reduction, with the redox ratio decreasing to 75.6% +/- 4.1% of baseline. The UnT and CsAr groups deceased to 67.1% +/- 4.0% and 67.2% +/- 3.6%, respectively (p < 0.005). During reperfusion the UnT group redox ratio increased to 1.59 +/- 0.04 times baseline. This increase was blunted in the CsAp (1.17 +/- 0.04, p = 0.026) and CsAr (1.35 +/- 0.02, p = 0.056) groups. Electron microscopy revealed reduced mitochondrial disruption in CsAp (19.7% +/- 7.6%) and CsAr (18.1% +/- 7.1%) rabbits compared with UnT (53.3% +/- 12.5%).
conclusionsFluorometric spectroscopy can be used in vivo to quantitatively assess the time course of CsA's influence on the mitochondrial dysfunction associated with myocardial ischemia and reperfusion.
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