ArticleBiomaterials2010
Chitosan-alginate 3D scaffolds as a mimic of the glioma tumor microenvironment.
Article in Biomaterials, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
65 citing papers in PubMed.
- Vascularization ability of glioma stem cells in different three-dimensional microenvironments.Regenerative biomaterials · 2024Article
- Biomaterial-basedCancer pathogenesis and therapy · 2023Review
- 3D Porous Scaffold-Based High-Throughput Platform for Cancer Drug Screening.Pharmaceutics · 2023Article
- Chitosan Cryogels Cross-Linked with 1,1,3-Triglycidyloxypropane: Mechanical Properties and Cytotoxicity for Cancer Cell 3D Cultures.Biomimetics (Basel, Switzerland) · 2023Article
- Article
- Review
- Assessing Polysaccharides/Aloe Vera-Based Hydrogels for Tumor Spheroid Formation.Gels (Basel, Switzerland) · 2023Article
- Preparation and characterization of a novel triple composite scaffold containing silk fibroin, chitosan, extracellular matrix and the mechanism of Akt/FoxO signaling pathway in colonic cancer cells cultured in 3D.Frontiers in bioengineering and biotechnology · 2023Article
- Organ Chips and Visualization of Biological Systems.Advances in experimental medicine and biology · 2023Article
- Machine learning and 3D bioprinting.International journal of bioprinting · 2023Article
- High-Throughput Dispensing of Viscous Solutions for Biomedical Applications.Micromachines · 2022Article
- Alginate-Based Bio-Composites and Their Potential Applications.Journal of functional biomaterials · 2022Review
- Glioblastoma, from disease understanding towards optimal cell-based in vitro models.Cellular oncology (Dordrecht, Netherlands) · 2022Review
- Article
- Biophysics Role and Biomimetic Culture Systems of ECM Stiffness in Cancer EMT.Global challenges (Hoboken, NJ) · 2022Review
- Polymeric Hydrogels for In Vitro 3D Ovarian Cancer Modeling.International journal of molecular sciences · 2022Review
- Strategies for developing complex multi-component in vitro tumor models: Highlights in glioblastoma.Advanced drug delivery reviews · 2022Review
- Three-Dimensional Cell Culture Models of Hepatocellular Carcinoma - a Review.Journal of gastrointestinal cancer · 2021Review
- Reconstructing the tumor architecture into organoids.Advanced drug delivery reviews · 2021Review
- Cancer Cell Direct Bioprinting: A Focused Review.Micromachines · 2021Review
5 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
Despite recent advances in the understanding of its cell biology, glioma remains highly lethal. Development of effective therapies requires a cost-effective in vitro tumor model that more accurately resembles the in vivo tumor microenvironment as standard two-dimensional (2D) tissue culture conditions do so poorly. Here we report on the use of a three-dimensional (3D) chitosan-alginate (CA) scaffold to serve as an extracellular matrix that promotes the conversion of cultured cancer cells to a more malignant in vivo-like phenotype. Human U-87 MG and U-118 MG glioma cells and rat C6 glioma cells were chosen for the study. In vitro tumor cell proliferation and secretion of factors that promote tumor malignancy, including VEGF, MMP-2, fibronectin, and laminin, were assessed. The scaffolds pre-cultured with U-87 MG and C6 cells were then implanted into nude mice to evaluate tumor growth and blood vessel recruitment compared to the standard 2D cell culture and 3D Matrigel matrix xenograft controls. Our results indicate that while the behavior of C6 cells showed minimal differences due to their highly malignant and invasive nature, U-87 MG and U-118 MG cells exhibited notably higher malignancy when cultured in CA scaffolds. CA scaffolds provide a 3D microenvironment for glioma cells that is more representative of the in vivo tumor, thus can serve as a more effective platform for development and study of anticancer therapeutics. This unique CA scaffold platform may offer a valuable alternative strategy to the time-consuming and costly animal studies for a wide variety of experimental designs.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.