ArticleCurrent biology : CB2010
Proteolytic instability and the action of nonclassical transcriptional activators.
Article in Current biology : CB, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Article
- Engineering transcription factor-based biosensors for repressive regulation through transcriptional deactivation design in Saccharomyces cerevisiae.Microbial cell factories · 2020Article
- Chromatin Association of Gcn4 Is Limited by Post-translational Modifications Triggered by its DNA-Binding in Saccharomyces cerevisiae.Genetics · 2016Article
- Sequential posttranslational modifications regulate PKC degradation.Molecular biology of the cell · 2016Article
- Fungal mediator tail subunits contain classical transcriptional activation domains.Molecular and cellular biology · 2015Article
- Functions of the proteasome on chromatin.Biomolecules · 2014Review
- The chemistry of regulation of genes and other things.The Journal of biological chemistry · 2014Review
- Molecular mechanisms of gonadotropin-releasing hormone signaling: integrating cyclic nucleotides into the network.Frontiers in endocrinology · 2013Review
- Proteasome-mediated turnover of Arabidopsis MED25 is coupled to the activation of FLOWERING LOCUS T transcription.Plant physiology · 2012Article
- Regulation of gene expression by the ubiquitin-proteasome system.Seminars in cell & developmental biology · 2012Review
- Mediator acts upstream of the transcriptional activator Gal4.PLoS biology · 2012Article
- Ubiquitin and proteasomes in transcription.Annual review of biochemistry · 2012Review
- Transcriptional activators and activation mechanisms.Protein & cell · 2011Review
- Review
- An effect of DNA sequence on nucleosome occupancy and removal.Nature structural & molecular biology · 2011Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Several transcriptional activators, called "classical" because each bears a natural acidic activating region attached to a DNA binding domain, are proteolytically unstable in yeast, and it has been suggested that this instability is required for transcriptional activation. Here we test the generality of that proposal by examining a set of activators (called "nonclassical") that lack activating regions. These activators (e.g., LexA-Gal11) comprise a LexA DNA binding domain fused to a component of the Mediator and are believed to insert the latter into the Mediator and recruit it (and, indirectly, other components required for transcription) to a gene bearing LexA sites. We find that three, and only three, Mediator subunits, all from its tail domain, work as activators when fused to LexA. All three are unstable, and for the case analyzed in detail, stabilization decreases activity. Thus, to the extent tested, both classical and nonclassical activators work most efficiently when proteolytically unstable.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.