ArticleBMC microbiology2010
Cell-associated hemolysis activity in the clinical strain of Pseudomonas fluorescens MFN1032.
Article in BMC microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed.
- Hemolytic mechanism and virulence of Antarctic psychrophilicFrontiers in microbiology · 2026Article
- In vitro virulence activity of Pseudomonas aeruginosa, enhanced by either Acinetobacter baumannii or Enterococcus faecium through the polymicrobial interactions.Archives of microbiology · 2022Article
- Biofilm and Pathogenesis-Related Proteins in the FoodborneFrontiers in microbiology · 2020Article
- Synthesis and antibiotic activity of novel acylated phloroglucinol compounds against methicillin-resistant Staphylococcus aureus.The Journal of antibiotics · 2019Article
- Competitive Fitness of Essential Gene Knockdowns Reveals a Broad-Spectrum Antibacterial Inhibitor of the Cell Division Protein FtsZ.Antimicrobial agents and chemotherapy · 2018Article
- Draft Genome Sequence of Carbapenem-Resistant Pseudomonas fluorescens Strain BWKM6, Isolated from Feces of Mareca penelope.Genome announcements · 2018Article
- Nod2: The intestinal gate keeper.PLoS pathogens · 2017Review
- Encapsulation ofInternational journal of nanomedicine · 2017Article
- Comparative genomics of Pseudomonas fluorescens subclade III strains from human lungs.BMC genomics · 2015Article
- Type III secretion system and virulence markers highlight similarities and differences between human- and plant-associated pseudomonads related to Pseudomonas fluorescens and P. putida.Applied and environmental microbiology · 2015Article
- A Pipeline for Screening Small Molecules with Growth Inhibitory Activity against Burkholderia cenocepacia.PloS one · 2015Article
- Microbiology, genomics, and clinical significance of the Pseudomonas fluorescens species complex, an unappreciated colonizer of humans.Clinical microbiology reviews · 2014Review
- Propensity to high-fat diet-induced obesity in mice is associated with the indigenous opportunistic bacteria on the interior of Peyer's patches.Journal of clinical biochemistry and nutrition · 2014Article
- Virulence of the Pseudomonas fluorescens clinical strain MFN1032 towards Dictyostelium discoideum and macrophages in relation with type III secretion system.BMC microbiology · 2012Article
- Structural and functional analysis of the type III secretion system from Pseudomonas fluorescens Q8r1-96.Journal of bacteriology · 2011Article
- Pseudomonas fluorescens alters epithelial permeability and translocates across Caco-2/TC7 intestinal cells.Gut pathogens · 2010Article
- The clinical Pseudomonas fluorescens MFN1032 strain exerts a cytotoxic effect on epithelial intestinal cells and induces Interleukin-8 via the AP-1 signaling pathway.BMC microbiology · 2010Article
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7 authors.
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Abstract
backgroundMFN1032 is a clinical Pseudomonas fluorescens strain able to grow at 37 degrees C. MFN1032 cells induce necrosis and apoptosis in rat glial cells at this temperature. This strain displays secretion-mediated hemolytic activity involving phospholipase C and cyclolipopeptides. Under laboratory conditions, this activity is not expressed at 37 degrees C. This activity is tightly regulated and is subject to phase variation.
resultsWe found that MFN1032 displays a cell-associated hemolytic activity distinct from the secreted hemolytic activity. Cell-associated hemolysis was expressed at 37 degrees C and was only detected in vitro in mid log growth phase in the presence of erythrocytes. We studied the regulation of this activity in the wild-type strain and in a mutant defective in the Gac two-component pathway. GacS/GacA is a negative regulator of this activity. In contrast to the Pseudomonas fluorescens strains PfO-1 and Pf5, whose genomes have been sequenced, the MFN1032 strain has the type III secretion-like genes hrcRST belonging to the hrpU operon. We showed that disruption of this operon abolished cell-associated hemolytic activity. This activity was not detected in P.fluorescens strains carrying similar hrc genes, as for the P. fluorescens psychrotrophic strain MF37.
conclusionsTo our knowledge this the first demonstration of cell-associated hemolytic activity of a clinical strain of Pseudomonas fluorescens. Moreover, this activity seems to be related to a functional hrpU operon and is independent of biosurfactant production. Precise link between a functional hrpU operon and cell-associated hemolytic activity remains to be elucidated.
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