ArticleThe Journal of molecular diagnostics : JMD2010
Immunoguided laser assisted microdissection techniques for DNA methylation analysis of archival tissue specimens.
Article in The Journal of molecular diagnostics : JMD, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 36 citations in OpenAlex.
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- An Improved Method for Cell Type-Selective Glycomic Analysis of Tissue Sections Assisted by Fluorescence Laser Microdissection.International journal of molecular sciences · 2019Article
- Computer-Aided Laser Dissection: A Microdissection Workflow Leveraging Image Analysis Tools.Journal of pathology informatics · 2018Article
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- Lymphomatoid granulomatosis--a single institute experience: pathologic findings and clinical correlations.The American journal of surgical pathology · 2015Article
- SIVQ-LCM protocol for the ArcturusXT instrument.Journal of visualized experiments : JoVE · 2014Article
- Early lesions of follicular lymphoma: a genetic perspective.Haematologica · 2014Article
- Comparison of clinical targeted next-generation sequence data from formalin-fixed and fresh-frozen tissue specimens.The Journal of molecular diagnostics : JMD · 2013Article
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- Nodal involvement by cutaneous CD30-positive T-cell lymphoma mimicking classical Hodgkin lymphoma.The American journal of surgical pathology · 2012Article
- Semiautomated laser capture microdissection of lung adenocarcinoma cytology samples.Acta cytologica · 2012Article
- Clonally related histiocytic/dendritic cell sarcoma and chronic lymphocytic leukemia/small lymphocytic lymphoma: a study of seven cases.Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc · 2011Article
- Differential vitamin D 24-hydroxylase/CYP24A1 gene promoter methylation in endothelium from benign and malignant human prostate.Epigenetics · 2011Article
- Why is it crucial to reintegrate pathology into cancer research?BioEssays : news and reviews in molecular, cellular and developmental biology · 2011Article
- Effect of immunohistochemistry on molecular analysis of tissue samples: implications for microdissection technologies.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2011Article
- Coexisting and clonally identical classic hodgkin lymphoma and nodular lymphocyte predominant hodgkin lymphoma.The American journal of surgical pathology · 2011Article
- Expression microdissection adapted to commercial laser dissection instruments.Nature protocols · 2011Article
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Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Altered DNA methylation is a fundamental characteristic of carcinogenesis. The analysis of DNA methylation in tumor cells may help to better understand tumor pathogenesis and more importantly may be used as diagnostic tool with therapeutic consequences. To detect targets relevant in tumorigenesis, it is essential to separate neoplastic cells from nonneoplastic cells. An excellent method for isolating specific cells is laser-assisted microdissection (LAM). Target cell identification for immunoguided LAM (ILAM) requires immunohistochemistry (IHC). Yet, it is unclear whether IHC for ILAM influences DNA methylation. The goals of this study were to establish an optimized protocol for antigen retrieval and IHC of formalin-fixed paraffin-embedded (FFPE) specimens suitable for ILAM and to evaluate its effect on the DNA methylome using a high throughput array. Using ten archival FFPE specimens, we showed specific staining suitable for ILAM. Extracted DNA from microdissected cells of immunohistochemically or H&E-stained tissue sections showed identical DNA quality and a strong correlation (r = 0.94 to 0.98) for CpG target methylation of 1505 analyzed sites in a series of five paired samples. No differential methylation between H&E and IHC was detected in 1501 of 1505 CpG targets (99.7%; P < 0.05). These results demonstrate the validity and utility of the herein described protocol, which allows the application of ILAM for large-scale genomic and epigenetic analyses of archival tissue specimens.
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Registered trials
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