ArticleThe journal of sexual medicine2010
Endothelin-1 induces contraction of female rat internal pudendal and clitoral arteries through ET(A) receptor and rho-kinase activation.
Article in The journal of sexual medicine, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 19 citations in OpenAlex.
- The effect of mirabegron, used for overactive bladder treatment, on female sexual function: a prospective controlled study.BMC urology · 2018Trial
- Functions of RhoA in the female reproductive system.Reproductive and developmental medicine · 2025Review
- Alterations in the phosphodiesterase type 5 pathway and oxidative stress correlate with erectile function in spontaneously hypertensive rats.Journal of cellular and molecular medicine · 2020Article
- Overactive Bladder Syndrome Treatments and Their Effect on Female Sexual Function: A Review.Sexual medicine · 2020Review
- Impact of Immune System Activation and Vascular Impairment on Male and Female Sexual Dysfunction.Sexual medicine reviews · 2019Review
- Investigation of the association between Rho/Rho-kinase gene polymorphisms and systemic sclerosis.Rheumatology international · 2016Article
- Cross-talk between endothelin-1 and mineral metabolism in hemodialysis patients: a cross-sectional study.Iranian Red Crescent medical journal · 2014Article
- Prevention of RhoA activation and cofilin-mediated actin polymerization mediates the antihypertrophic effect of adenosine receptor agonists in angiotensin II- and endothelin-1-treated cardiomyocytes.Molecular and cellular biochemistry · 2014Article
- New insights into hypertension-associated erectile dysfunction.Current opinion in nephrology and hypertension · 2012Article
- Characterization of the vasculature supplying the genital tissues in female rats.The journal of sexual medicine · 2012Article
- Internal pudendal artery from type 2 diabetic female rats demonstrate elevated endothelin-1-mediated constriction.The journal of sexual medicine · 2011Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
introductionEndothelin-1 (ET-1), a potent vasoconstrictor peptide, acts mainly through the Gprotein-coupled ET(A) receptor (ET(A)R). Increased vascular ET-1 production and constrictor sensitivity have been observed in various cardiovascular diseases, including hypertension, as well as erectile dysfunction. The internal pudendal artery (IPA) supplies blood to the vagina and clitoris. Inadequate blood flow through the IPA may lead to insufficient vaginal engorgement and clitoral tumescence.
aimCharacterize the effects of ET-1 on the IPA and clitoral artery (CA).
methodsIPA and CA from female Sprague Dawley rats (225-250 g) were mounted in myograph chambers. Arterial segments were submitted to increasing concentrations of ET-1 (10-10-10-6 M). Segments were incubated with the ET(A)R antagonist, atrasentan (10-8 M) or the Rho-kinase inhibitor, Y-27632 (10-6 M) 30 minutes prior to agonist exposure. All E(max) values are expressed as % KCl-induced maximal contraction. ET(A)R, RhoA, and Rho-kinase expression from IPA was evaluated by Western blot. mRNA of preproET-1, ET(A)R, ET(B)R, RhoA, and Rho-kinase were measured by real time PCR.
main outcome measuresET-1 constrictor sensitivity in IPA and CA, protein expression and messenger RNA levels of ET-1-mediated constriction components.
resultsET-1 concentration-dependently contracted IPA (% Contraction and pD2, respectively: 156 ± 18, 8.2 ± 0.1) and CA (163 ± 12, 8.8 ± 0.08), while ET(A)R antagonism reduced ET-1-mediated contraction (IPA: 104 ± 23, 6.4 ± 0.2; CA: 112 ± 17, 6.6 ± 0.08). Pretreatment with Y-27632 significantly shifted ET-1 pD2 in IPA (108 ± 24, 7.9 ± 0.1) and CA (147 ± 58 and 8.0 ± 0.25). Protein expression of ET(A)R, ET(B)R, RhoA, and Rho-kinase were detected in IPA. IPA and CA contained preproET-1, ET(A)R, ET(B)R, RhoA, and Rho-kinase message.
conclusionWe observed that the IPA and CA are sensitive to ET-1, signaling through the ET(A)R and Rho-kinase pathway. These data indicate that ET-1 may play a role in vaginal and clitoral blood flow and may be important in pathologies where ET-1 levels are elevated.
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