ArticleStem cell reviews and reports2010
Selective removal of undifferentiated embryonic stem cells from differentiation cultures through HSV1 thymidine kinase and ganciclovir treatment.
Article in Stem cell reviews and reports, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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Who cites it
20 citing papers in PubMed, 37 citations in OpenAlex.
- Regional random mutagenesis driven by multiple sgRNAs and diverse on-target genome editing events to identify functionally important elements in non-coding regions.Open biology · 2024Article
- Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system.Cell reports methods · 2023Article
- Strategies to Improve the Safety of iPSC-Derived β Cells for β Cell Replacement in Diabetes.Transplant international : official journal of the European Society for Organ Transplantation · 2022Review
- An Overview on Promising Somatic Cell Sources Utilized for the Efficient Generation of Induced Pluripotent Stem Cells.Stem cell reviews and reports · 2021Review
- Efficient Genetic Safety Switches for Future Application of iPSC-Derived Cell Transplants.Journal of personalized medicine · 2021Article
- Article
- Alpha-Herpesvirus Thymidine Kinase Genes Mediate Viral Virulence and Are Potential Therapeutic Targets.Frontiers in microbiology · 2019Review
- Hepatic population derived from human pluripotent stem cells is effectively increased by selective removal of undifferentiated stem cells using YM155.Stem cell research & therapy · 2017Article
- A Quick and Efficient Method for the Purification of Endoderm Cells Generated from Human Embryonic Stem Cells.Journal of visualized experiments : JoVE · 2016Article
- PI3K/AKT Signaling Pathway Is Essential for Survival of Induced Pluripotent Stem Cells.PloS one · 2016Article
- Cytotoxicity and activation of the Wnt/beta-catenin pathway in mouse embryonic stem cells treated with four GSK3 inhibitors.BMC research notes · 2014Article
- MicroRNA target sites as genetic tools to enhance promoter-reporter specificity for the purification of pancreatic progenitor cells from differentiated embryonic stem cells.Stem cell reviews and reports · 2013Article
- Targeting of herpes simplex virus 1 thymidine kinase gene sequences into the OCT4 locus of human induced pluripotent stem cells.PloS one · 2013Article
- Lentiviral vector mediated thymidine kinase expression in pluripotent stem cells enables removal of tumorigenic cells.PloS one · 2013Article
- A critical re-evaluation of CD24-positivity of human embryonic stem cells differentiated into pancreatic progenitors.Stem cell reviews and reports · 2012Article
- Stem cell and biomaterials research in dental tissue engineering and regeneration.Dental clinics of North America · 2012Review
- 'Hearts and bones': the ups and downs of 'plasticity' in stem cell biology.EMBO molecular medicine · 2012Review
- Article
- Insulin-producing surrogate β-cells from embryonic stem cells: are we there yet?Molecular therapy : the journal of the American Society of Gene Therapy · 2011Review
- Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pluripotent cell lines such as embryonic stem cells are an attractive source for a potential cell replacement therapy. However, transplantation of differentiated cells harbors the risk of teratoma formation, presenting a serious health risk. To overcome this obstacle, a negative selection system was established that permits selective removal of undifferentiated cells during in vitro differentiation. Use of the HSV1 thymidine kinase and eGFP under the control of the Oct4 promoter allowed the destruction of undifferentiated ES cells by ganciclovir treatment; differentiated cells were unharmed. Clonal ES cells remained pluripotent and showed positive staining for a wide range of embryonic markers. Thus, treatment with ganciclovir during in vitro differentiation effectively removed the population of undifferentiated cells and provided a pure population of completely differentiated cells. This approach may pave the way for a safe application of ES cells in regenerative medicine in the future.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.