ArticleGene therapy2010
Therapeutic levels of erythropoietin (EPO) achieved after gene electrotransfer to skin in mice.
Article in Gene therapy, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 36 citations in OpenAlex.
- Electroporation in Cancer Therapy: A Simplified Model Derived from the Hodgkin-Huxley Model.Bioelectricity · 2024Article
- Exosomes as an Emerging Plasmid Delivery Vehicle for Gene Therapy.Pharmaceutics · 2023Review
- Induction of a local muscular dystrophy using electroporation in vivo: an easy tool for screening therapeutics.Scientific reports · 2020Article
- Efficient delivery of nucleic acid molecules into skin by combined use of microneedle roller and flexible interdigitated electroporation array.Theranostics · 2018Article
- Direct Current Helium Plasma forPlasma medicine · 2017Article
- Electrotransfer parameters as a tool for controlled and targeted gene expression in skin.Molecular therapy. Nucleic acids · 2016Article
- Electroporation-delivered transdermal neostigmine in rats: equivalent action to intravenous administration.Drug design, development and therapy · 2016Article
- Improved Specificity of Gene Electrotransfer to Skin Using pDNA Under the Control of Collagen Tissue-Specific Promoter.The Journal of membrane biology · 2015Article
- What you always needed to know about electroporation based DNA vaccines.Human vaccines & immunotherapeutics · 2012Review
- Nonviral gene delivery of erythropoietin by mesenchymal stromal cells.Gene therapy · 2012Article
- Electro-gene transfer to skin using a noninvasive multielectrode array.Journal of controlled release : official journal of the Controlled Release Society · 2011Article
- Evaluation of delivery conditions for cutaneous plasmid electrotransfer using a multielectrode array.Gene therapy · 2011Article
- Duration and level of transgene expression after gene electrotransfer to skin in mice.Gene therapy · 2010Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gene electrotransfer refers to gene transfection by electroporation and is an effective non-viral method for delivering naked DNA into cells and tissues. This study presents data from gene electrotransfer with erythropoietin (EPO) to mouse skin. Nine-week-old female NMRI mice received one, two or three intradermal injections of 50 microg EPO plasmid and were subsequently electroporated. With plate electrodes and 100 microg of EPO, a significant increase in hemoglobin (P<0.01) was observed compared with controls. The level of hemoglobin peaked after 5 weeks but stayed significantly elevated for more than 3 months. Serum EPO was significantly increased (P<0.001) 24 h after the transfection and remained significantly different compared with controls until the maximum level of serum EPO was reached after 2 weeks. Eight weeks after the transfection serum EPO returned to baseline. In this study, we have established that gene electrotransfer to skin of even small amounts of DNA can lead to systemically therapeutic levels of protein. This means that in addition to DNA vaccinations, there is a potential utility for electroporation in alleviating systemic diseases such as cancer and protein deficiency disorders.
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