Evidence map›Paper›PMID 20410316›Full record

ArticleThe Journal of general virology2010

Interaction of the human cytomegalovirus uracil DNA glycosylase UL114 with the viral DNA polymerase catalytic subunit UL54.

Blair L Strang, Donald M Coen

Open access · greenAbstract read
In one paragraph

Article in The Journal of general virology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
1.1field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 23 citations in OpenAlex.

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  13. Herpes simplex viruses: mechanisms of DNA replication.Cold Spring Harbor perspectives in biology · 2012
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Blair L StrangDepartment of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA.
Donald M CoenDepartment of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA.
Harvard University · US

Funding

Human Cytomegalovirus Nuclear Egress: Molecular Mechanisms and Drug TargetingR01AI026077 · NIAID · HARVARD UNIVERSITY (MEDICAL SCHOOL) · PI COEN, DONALD M · 1999 to 2019
$10.9M
MOLECULAR GENETICS OF HSV DNA POLYMERASE GENER01AI019838 · NIAID · HARVARD UNIVERSITY (MEDICAL SCHOOL) · PI COEN, DONALD M, HOGLE, JAMES M · 1985 to 2015
$5.9M
Human Cytomegalovirus Nuclear Egress: Molecular Mechanisms and Drug TargetingR56AI026077 · NIAID · HARVARD MEDICAL SCHOOL · PI COEN, DONALD M, HOGLE, JAMES M · 2009 to 2009
$532k
NIAID NIH HHS AI19838NIAID NIH HHS AI26077NIAID NIH HHS R01 AI019838NIAID NIH HHS R01 AI026077NIAID NIH HHS R56 AI026077
6 · The paper itself

Abstract

Interaction between human cytomegalovirus uracil DNA glycosylase (UL114) and the viral DNA polymerase accessory subunit (UL44) has been reported; however, no such association was found in proteomic studies of UL44-interacting proteins. Utilizing virus expressing FLAG-tagged UL114, nuclease-resistant association of UL44 and the DNA polymerase catalytic subunit UL54 with UL114 was observed by co-immunoprecipitation. Contrary to a previous report, we observed that UL114 was much less abundant than UL44. Interaction of UL114 with UL54, independent of the UL54 carboxyl terminus, but not with UL44 was detected in vitro. Our data are consistent with a direct UL114-UL54 interaction, and suggest that UL114 and UL54 act in concert during base excision repair of the viral genome.

Indexed as

Protein Interaction MappingCytomegalovirusDNA-Binding ProteinsDNA-Directed DNA PolymeraseHumansImmunoprecipitationProtein BindingUracil-DNA GlycosidaseViral ProteinsDNA-Binding ProteinsDNA-Directed DNA PolymeraseICP36 protein, CytomegalovirusUL54 protein, Human herpesvirus 5Uracil-DNA GlycosidaseViral Proteins

Identifiers

PMID20410316
PMCPMC3052538
OpenAlexW2016964717

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.