ArticleThe Journal of biological chemistry2010
Arabidopsis thaliana pattern recognition receptors for bacterial elongation factor Tu and flagellin can be combined to form functional chimeric receptors.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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47 citing papers in PubMed, 107 citations in OpenAlex.
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Morphology and Comparative Transcriptome Analysis of Resistant and Susceptible Bitter Gourd (Journal of fungi (Basel, Switzerland) · 2026Article
- Review
- Transcriptome Analysis of DAMP-Induced Root Growth Regulation and Defense in Foxtail Millet.International journal of molecular sciences · 2025Article
- Plant pattern recognition receptors: from evolutionary insight to engineering.Nature reviews. Genetics · 2025Review
- It's Complicated: Why Are There So Few Commercially Successful Crop Varieties Engineered for Disease Resistance?Molecular plant pathology · 2025Review
- Natural variation in the pattern-triggered immunity response in plants: Investigations, implications and applications.Molecular plant pathology · 2024Review
- Feeding Assay to Study the Effect of Phytocytokines on Direct and Indirect Defense in Maize.Methods in molecular biology (Clifton, N.J.) · 2024Article
- A large-scale screening identifies receptor-like kinases with common features in kinase domains that are potentially related to disease resistance in planta.Frontiers in plant science · 2024Article
- The Arabidopsis MIK2 receptor elicits immunity by sensing a conserved signature from phytocytokines and microbes.Nature communications · 2021Article
- Genome editing for resistance against plant pests and pathogens.Transgenic research · 2021Review
- Perception of unrelated microbe-associated molecular patterns triggers conserved yet variable physiological and transcriptional changes in Brassica rapa ssp. pekinensis.Horticulture research · 2020Article
- Molecular Characterization of Differences between the Tomato Immune Receptors Flagellin Sensing 3 and Flagellin Sensing 2.Plant physiology · 2020Article
- The BIR2/BIR3-Associated Phospholipase Dγ1 Negatively Regulates Plant Immunity.Plant physiology · 2020Article
- Deviating from the Beaten Track: New Twists in Brassinosteroid Receptor Function.International journal of molecular sciences · 2020Review
- Article
- An EFR-Cf-9 chimera confers enhanced resistance to bacterial pathogens by SOBIR1- and BAK1-dependent recognition of elf18.Molecular plant pathology · 2019Article
- Pattern recognition receptors and their interactions with bacterial type III effectors in plants.Genes & genomics · 2019Review
- Comparing Arabidopsis receptor kinase and receptor protein-mediated immune signaling reveals BIK1-dependent differences.The New phytologist · 2019Article
- Screening and identification of a non-peptide antagonist for the peptide hormone receptor inCommunications biology · 2019Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The receptor kinase EFR of Arabidopsis thaliana detects the microbe-associated molecular pattern elf18, a peptide that represents the N terminus of bacterial elongation factor Tu. Here, we tested subdomains of EFR for their importance in receptor function. Transient expression of tagged versions of EFR and EFR lacking its cytoplasmic domain in leaves of Nicotiana benthamiana resulted in functional binding sites for elf18. No binding of ligand was found with the ectodomain lacking the transmembrane domain or with EFR lacking the first 5 of its 21 leucine-rich repeats (LRRs). EFR is structurally related to the receptor kinase flagellin-sensing 2 (FLS2) that detects bacterial flagellin. Chimeric receptors with subdomains of FLS2 substituting for corresponding parts of EFR were tested for functionality in ligand binding and receptor activation assays. Substituting the transmembrane domain and the cytoplasmic domain resulted in a fully functional receptor for elf18. Replacing also the outer juxtamembrane domain with that of FLS2 led to a receptor with full affinity for elf18 but with a lower efficiency in response activation. Extending the substitution to encompass also the last two of the LRRs abolished binding and receptor activation. Substitution of the N terminus by the first six LRRs from FLS2 reduced binding affinity and strongly affected receptor activation. In summary, chimeric receptors allow mapping of subdomains relevant for ligand binding and receptor activation. The results also show that modular assembly of chimeras from different receptors can be used to form functional receptors.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.