ArticleMolecular biology of the cell2010
Mug28, a meiosis-specific protein of Schizosaccharomyces pombe, regulates spore wall formation.
Article in Molecular biology of the cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 8 citations in OpenAlex.
- Genome-wide quantification of contributions to sexual fitness identifies genes required for spore viability and health in fission yeast.PLoS genetics · 2022Article
- Spore Germination Requires Ferrichrome Biosynthesis and the Siderophore Transporter Str1 inGenetics · 2019Article
- A role for the transcription factor Mca1 in activating the meiosis-specific copper transporter Mfc1.PloS one · 2018Article
- Php4 Is a Key Player for Iron Economy in Meiotic and Sporulating Cells.G3 (Bethesda, Md.) · 2016Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
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Abstract
The meiosis-specific mug28(+) gene of Schizosaccharomyces pombe encodes a putative RNA-binding protein with three RNA recognition motifs (RRMs). Live observations of meiotic cells that express Mug28 tagged with green fluorescent protein (GFP) revealed that Mug28 is localized in the cytoplasm, and accumulates around the nucleus from metaphase I to anaphase II. Disruption of mug28(+) generated spores with low viability, due to the aberrant formation of the forespore membrane (FSM). Visualization of the FSM in living cells expressing GFP-tagged Psy1, an FSM protein, indicated that mug28Delta cells harbored abnormal FSMs that contained buds, and had a delayed disappearance of Meu14, a leading edge protein. Electron microscopic observation revealed that FSM formation was abnormal in mug28Delta cells, showing bifurcated spore walls that were thicker than the nonbifurcated spore walls of the wild type. Analysis of Mug28 mutants revealed that RRM3, in particular phenylalanin-466, is of primary importance for the proper localization of Mug28, spore viability, and FSM formation. Together, we conclude that Mug28 is essential for the proper maturation of the FSM and the spore wall.
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