ArticleMolecular biology of the cell2010
Promoter DNA methylation patterns of differentiated cells are largely programmed at the progenitor stage.
Article in Molecular biology of the cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
43 citing papers in PubMed, 1 synthesis or guideline pooled it, 80 citations in OpenAlex.
- Pooled it
- Semiquantitative Cardiac-Specific Gene Expression Validation of the DNA Methylation Microarray in Human Mesenchymal Stem Cells.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Epigenetic control of skeletal muscle atrophy.Cellular & molecular biology letters · 2024Review
- The Molecular Regulatory Mechanism in Multipotency and Differentiation of Wharton's Jelly Stem Cells.International journal of molecular sciences · 2023Review
- Temporal Modulation of DNA Methylation and Gene Expression in Monolayer and 3D Spheroids of Dental Pulp Stem Cells during Osteogenic Differentiation: A Comparative Study.Tissue engineering and regenerative medicine · 2022Article
- Local euchromatin enrichment in lamina-associated domains anticipates their repositioning in the adipogenic lineage.Genome biology · 2022Article
- Role of epigenetics in cellular reprogramming; from iPSCs to disease modeling and cell therapy.Journal of cellular biochemistry · 2022Review
- 3D Tissue-Engineered Vascular Drug Screening Platforms: Promise and Considerations.Frontiers in cardiovascular medicine · 2022Review
- Genome-wide methylome pattern predictive network analysis reveal mesenchymal stem cell's propensity to undergo cardiovascular lineage.3 Biotech · 2022Article
- The implications of IDH mutations for cancer development and therapy.Nature reviews. Clinical oncology · 2021Review
- Article
- New Challenge: Mitochondrial Epigenetics?Stem cell reviews and reports · 2018Review
- Influence of maternal obesity, diet and exercise on epigenetic regulation of adipocytes.Molecular aspects of medicine · 2017Review
- INO80 is Required for Osteogenic Differentiation of Human Mesenchymal Stem Cells.Scientific reports · 2016Article
- Epigenetic Mechanisms Regulating Mesenchymal Stem Cell Differentiation.Current genomics · 2015Article
- Genome-wide analysis of DNA methylation and gene expression patterns in purified, uncultured human liver cells and activated hepatic stellate cells.Oncotarget · 2015Article
- Impact of tissue-specific stem cells on lineage-specific differentiation: a focus on the musculoskeletal system.Stem cell reviews and reports · 2015Review
- Epigenetic changes during mechanically induced osteogenic lineage commitment.Journal of biomechanical engineering · 2015Article
- Impact of source tissue and ex vivo expansion on the characterization of goat mesenchymal stem cells.Journal of animal science and biotechnology · 2015Article
- DNA methylation dynamics in muscle development and disease.Frontiers in aging neuroscience · 2015Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mesenchymal stem cells (MSCs) isolated from various tissues share common phenotypic and functional properties. However, intrinsic molecular evidence supporting these observations has been lacking. Here, we unravel overlapping genome-wide promoter DNA methylation patterns between MSCs from adipose tissue, bone marrow, and skeletal muscle, whereas hematopoietic progenitors are more epigenetically distant from MSCs as a whole. Commonly hypermethylated genes are enriched in signaling, metabolic, and developmental functions, whereas genes hypermethylated only in MSCs are associated with early development functions. We find that most lineage-specification promoters are DNA hypomethylated and harbor a combination of trimethylated H3K4 and H3K27, whereas early developmental genes are DNA hypermethylated with or without H3K27 methylation. Promoter DNA methylation patterns of differentiated cells are largely established at the progenitor stage; yet, differentiation segregates a minor fraction of the commonly hypermethylated promoters, generating greater epigenetic divergence between differentiated cell types than between their undifferentiated counterparts. We also show an effect of promoter CpG content on methylation dynamics upon differentiation and distinct methylation profiles on transcriptionally active and inactive promoters. We infer that methylation state of lineage-specific promoters in MSCs is not a primary determinant of differentiation capacity. Our results support the view of a common origin of mesenchymal progenitors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.