ArticleMethods in cell biology2009
Total internal reflection fluorescence (TIRF) microscopy of Chlamydomonas flagella.
Article in Methods in cell biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 48 citations in OpenAlex.
- Article
- Distribution and bulk flow analyses of the intraflagellar transport (IFT) motor kinesin-2 support an "on-demand" model for Chlamydomonas ciliary length control.Cytoskeleton (Hoboken, N.J.) · 2024Article
- Review
- Loss of ARL13 impedes BBSome-dependent cargo export from Chlamydomonas cilia.The Journal of cell biology · 2022Article
- Conversion of anterograde into retrograde trains is an intrinsic property of intraflagellar transport.Current biology : CB · 2022Article
- In vivo imaging shows continued association of several IFT-A, IFT-B and dynein complexes while IFT trains U-turn at the tip.Journal of cell science · 2021Article
- Potential Therapeutic Targets for Olfactory Dysfunction in Ciliopathies Beyond Single-Gene Replacement.Chemical senses · 2021Review
- Detachment ofSensors (Basel, Switzerland) · 2020Article
- Non-ciliary Roles of IFT Proteins in Cell Division and Polycystic Kidney Diseases.Frontiers in cell and developmental biology · 2020Review
- Article
- Article
- Chromosome-level genome assembly and transcriptome of the green algaProceedings of the National Academy of Sciences of the United States of America · 2017Article
- The Intraflagellar Transport Machinery.Cold Spring Harbor perspectives in biology · 2016Review
- The role of the dynein light intermediate chain in retrograde IFT and flagellar function in Chlamydomonas.Molecular biology of the cell · 2016Article
- Dynein and intraflagellar transport.Experimental cell research · 2015Review
- FAP206 is a microtubule-docking adapter for ciliary radial spoke 2 and dynein c.Molecular biology of the cell · 2015Article
- Total internal reflection fluorescence microscopy of intraflagellar transport in Tetrahymena thermophila.Methods in cell biology · 2015Article
- Processive cytoskeletal motors studied with single-molecule fluorescence techniques.FEBS letters · 2014Review
- Flipping a phosphate switch on kinesin-II to turn IFT around.Developmental cell · 2014Article
- A differential cargo-loading model of ciliary length regulation by IFT.Current biology : CB · 2013Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The eukaryotic flagellum is host to a variety of dynamic behaviors, including flagellar beating, the motility of glycoproteins in the flagellar membrane, and intraflagellar transport (IFT), the bidirectional traffic of protein particles between the flagellar base and tip. IFT is of particular interest, as it plays integral roles in flagellar length control, cell signaling, development, and human disease. However, our ability to understand dynamic flagellar processes such as IFT is limited in large part by the fidelity with which we can image these behaviors in living cells. This chapter introduces the application of total internal reflection fluorescence (TIRF) microscopy to visualize the flagella of Chlamydomonas reinhardtii. The advantages and challenges of TIRF are discussed in comparison to confocal and differential interference contrast techniques. This chapter also reviews current IFT insights gleaned from TIRF microscopy of Chlamydomonas and provides an outlook on the future of the technique, with particular emphasis on combining TIRF with other emerging imaging technologies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.