ArticleMethods in cell biology2009
Recording and analyzing IFT in Chlamydomonas flagella.
Article in Methods in cell biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Intraflagellar transport speed is sensitive to genetic and mechanical perturbations to flagellar beating.The Journal of cell biology · 2024Article
- Testing the time-of-flight model for flagellar length sensing.Molecular biology of the cell · 2017Article
- Article
- The role of retrograde intraflagellar transport in flagellar assembly, maintenance, and function.The Journal of cell biology · 2012Article
- An outer arm Dynein conformational switch is required for metachronal synchrony of motile cilia in planaria.Molecular biology of the cell · 2010Article
- CEP290 tethers flagellar transition zone microtubules to the membrane and regulates flagellar protein content.The Journal of cell biology · 2010Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The transport of materials to and from the cell body and tips of eukaryotic flagella and cilia is carried out by a process called intraflagellar transport, or IFT. This process is essential for the assembly and maintenance of cilia and flagella: in the absence of IFT, cilia cannot assemble and, if IFT is arrested in ciliated cells, the cilia disassemble. The major IFT complex proteins and the major motor proteins, kinesin-2 and osm-3 (which transport particles from the cell body to ciliary tips) and cytoplasmic dynein 1b (which transports particles from ciliary tips to the cell body) have been identified. However, we have little understanding of the structure of the IFT particles, the cargo that these particles carry, how cargo is loaded and unloaded from the particles, or how the motor proteins are regulated. The focus of this chapter is to provide methods to observe and quantify the movements of IFT particles in Chlamydomonas flagella. IFT movements can be visualized in paralyzed or partially arrested flagella using either differential interference contrast (IFT) microscopy or, in cells with fluorescently tagged IFT components, with fluorescence microscopy. Methods for recording IFT movements and analyzing movements using kymograms are described.
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Registered trials
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