Evidence map›Paper›PMID 20409815›Full record

ArticleMethods in cell biology2009

Genetic and phenotypic analysis of flagellar assembly mutants in Chlamydomonas reinhardtii.

Carlo Iomini, Jacob E Till, Susan K Dutcher

Abstract read
In one paragraph

Article in Methods in cell biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Article
  2. Structural organization of the intermediate and light chain complex of Chlamydomonas ciliary I1 dynein.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021
    Article
  3. Article
  4. Proteases Shape theProteomes · 2018
    Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Carlo IominiDepartment of Developmental and Regenerative Biology, Mt. Sinai School of Medicine, New York, New York 10023, USA.
Jacob E Till
Susan K Dutcher

Funding

GENETIC ANALYSIS OF BASAL BODY FUNCTIONR01GM032843 · NIGMS · WASHINGTON UNIVERSITY · PI DUTCHER, SUSAN K · 1985 to 2018
$5.9M
NIGMS NIH HHS R01 GM032843
6 · The paper itself

Abstract

Conditional mutants for flagellar assembly (fla) provide a useful tool to study intraflagellar transport (IFT) at the molecular level, and provide a unique set of tools to analyze cilia. The analysis of IFT phenotypes of fla mutants at the permissive temperature by a quantitative image analysis approach identified four distinct phases of the IFT cycle and directly demonstrated structural and functional remodeling of IFT particles at both axonemal extremities. In addition, the genetic analysis of fla mutants reveal interesting interactions among genes involved in flagellar assembly that help to provide information about the structure and function of IFT particles and their motors. This chapter provides protocols to isolate, characterize, and identify conditional Chlamydomonas flagellar assembly mutants and their genes and to test genetic interactions among proteins encoded by these genes.

Indexed as

Chlamydomonas reinhardtiiFlagellaProtozoan ProteinsAnimalsGenetic Complementation TestGenetic LinkageGenotypeMolecular Motor ProteinsMutagenesisMutationPhenotypeMolecular Motor ProteinsProtozoan Proteins

Identifiers

PMID20409815
PMCPMC4090777

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.