ArticleMethods in cell biology2009
Approaches for functional analysis of flagellar proteins in African trypanosomes.
Article in Methods in cell biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 41 citations in OpenAlex.
- CryoEM structure of thebioRxiv : the preprint server for biology · 2025Article
- FAP20 is required for flagellum assembly inMolecular biology of the cell · 2024Article
- FAP20 is required for flagellum assembly inbioRxiv : the preprint server for biology · 2024Article
- FAP106 is an interaction hub for assembling microtubule inner proteins at the cilium inner junction.Nature communications · 2023Article
- Light chain 2 is a Tctex-type related axonemal dynein light chain that regulates directional ciliary motility in Trypanosoma brucei.PLoS pathogens · 2022Article
- PCD Genes-From Patients to Model Organisms and Back to Humans.International journal of molecular sciences · 2022Review
- APEX2 Proximity Proteomics Resolves Flagellum Subdomains and Identifies Flagellum Tip-Specific Proteins in Trypanosoma brucei.mSphere · 2021Article
- Article
- Mutations in TTC29, Encoding an Evolutionarily Conserved Axonemal Protein, Result in Asthenozoospermia and Male Infertility.American journal of human genetics · 2019Article
- A Homozygous Ancestral SVA-Insertion-Mediated Deletion in WDR66 Induces Multiple Morphological Abnormalities of the Sperm Flagellum and Male Infertility.American journal of human genetics · 2018Article
- Parasite motility is critical for virulence of African trypanosomes.Scientific reports · 2018Article
- Motility-based label-free detection of parasites in bodily fluids using holographic speckle analysis and deep learning.Light, science & applications · 2018Article
- The iron-dependent mitochondrial superoxide dismutase SODA promotesThe Journal of biological chemistry · 2017Article
- A Trypanosomatid Iron Transporter that Regulates Mitochondrial Function Is Required for Leishmania amazonensis Virulence.PLoS pathogens · 2016Article
- Cell Surface Proteomics Provides Insight into Stage-Specific Remodeling of the Host-Parasite Interface in Trypanosoma brucei.Molecular & cellular proteomics : MCP · 2015Article
- Knockdown of Inner Arm Protein IC138 in Trypanosoma brucei Causes Defective Motility and Flagellar Detachment.PloS one · 2015Article
- Insect stage-specific adenylate cyclases regulate social motility in African trypanosomes.Eukaryotic cell · 2015Article
- Insect stage-specific receptor adenylate cyclases are localized to distinct subdomains of the Trypanosoma brucei Flagellar membrane.Eukaryotic cell · 2014Article
- CMF22 is a broadly conserved axonemal protein and is required for propulsive motility in Trypanosoma brucei.Eukaryotic cell · 2013Article
- Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The eukaryotic flagellum is a highly conserved organelle serving motility, sensory, and transport functions. Although genetic, genomic, and proteomic studies have led to the identification of hundreds of flagellar and putative flagellar proteins, precisely how these proteins function individually and collectively to drive flagellum motility and other functions remains to be determined. In this chapter we provide an overview of tools and approaches available for studying flagellum protein function in the protozoan parasite Trypanosoma brucei. We begin by outlining techniques for in vitro cultivation of both T. brucei life cycle stages, as well as transfection protocols for the delivery of DNA constructs. We then describe specific assays used to assess flagellum function including flagellum preparation and quantitative motility assays. We conclude the chapter with a description of molecular genetic approaches for manipulating gene function. In summary, the availability of potent molecular tools, as well as the health and economic relevance of T. brucei as a pathogen, combine to make the parasite an attractive and integral experimental system for the functional analysis of flagellar proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.