ArticleBiophysical journal2010
Gramicidin channels are internally gated.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Membrane Elastic Deformations Modulate Gramicidin A Transbilayer Dimerization and Lateral Clustering.Biophysical journal · 2018Article
- Design, synthesis, and biological evaluation of stable βEuropean journal of medicinal chemistry · 2018Article
- Exchange of Gramicidin between Lipid Bilayers: Implications for the Mechanism of Channel Formation.Biophysical journal · 2017Article
- Small-molecule photostabilizing agents are modifiers of lipid bilayer properties.Biophysical journal · 2013Article
- The pH-dependent induction of lipid membrane ionic permeability by N-terminally lysine-substituted analogs of gramicidin A.European biophysics journal : EBJ · 2012Article
- Functional reconstitution of influenza A M2(22-62).Biochimica et biophysica acta · 2011Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Gramicidin channels are archetypal molecular subjects for solid-state NMR studies and investigations of single-channel or cation conductance. Until now, the transitions between on and off conductance states have been thought, based on multichannel studies, to represent monomer <--> dimer reactions. Here we use a single-molecule deposition method (vesicle fusion to a planar bilayer) to show that gramicidin dimer channels do not normally dissociate when conductance terminates. Furthermore, the observation of two 13C peaks in solid-state NMR indicates very stable dichotomous conformations for both the first and second peptide bonds in the monomers, and a two-dimensional chemical exchange spectrum with a 12-s mixing time demonstrates that the Val1 carbonyl conformations exchange slowly, with lifetimes of several seconds. It is proposed that gramicidin channels are gated by small conformational changes in the channel near the permeation pathway. These studies demonstrate how regulation of conformations governing closed <--> open transitions may be achieved and studied at the molecular level.
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Registered trials
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