ArticleStem cell reviews and reports2010
Culture conditions and signalling networks promoting the establishment of cell lines from parthenogenetic and biparental pig embryos.
Article in Stem cell reviews and reports, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
21 citing papers in PubMed, 69 citations in OpenAlex.
- Transcriptomic insights and feeder-free culturing of porcine expanded potential stem cells from cloned embryos.Stem cell research & therapy · 2025Article
- An optimized culture system for efficient derivation of porcine expanded potential stem cells from preimplantation embryos and by reprogramming somatic cells.Nature protocols · 2024Article
- Generation of Artificial Blastoids Combining miR-200-Mediated Reprogramming and Mechanical Cues.Cells · 2024Article
- Bioengineering-tissue strategies to model mammalian implantationFrontiers in bioengineering and biotechnology · 2024Article
- The progress of induced pluripotent stem cells derived from pigs: a mini review of recent advances.Frontiers in cell and developmental biology · 2024Review
- Tetraploid embryo aggregation produces high-quality blastocysts with an increased trophectoderm in pigs.Frontiers in cell and developmental biology · 2023Article
- Generation of Trophoblast-Like Cells From Hypomethylated Porcine Adult Dermal Fibroblasts.Frontiers in veterinary science · 2021Article
- Conversion between porcine naïve-like and primed ESCs and specific pluripotency marker identification.In vitro cellular & developmental biology. Animal · 2020Article
- New tools for cell reprogramming and conversion: Possible applications to livestock.Animal reproduction · 2019Article
- Establishment of porcine and human expanded potential stem cells.Nature cell biology · 2019Article
- Novel histone deacetylase inhibitors and embryo aggregation enhance cloned embryo development and ES cell derivation in pigs.PloS one · 2018Article
- Derivation of Porcine Embryonic Stem-Like Cells from In Vitro-Produced Blastocyst-Stage Embryos.Scientific reports · 2016Article
- Porcine Pluripotent Stem Cells Derived from IVF Embryos Contribute to Chimeric Development In Vivo.PloS one · 2016Article
- CRISPR/Cas9 as tool for functional study of genes involved in preimplantation embryo development.PloS one · 2015Article
- Putative porcine embryonic stem cell lines derived from aggregated four-celled cloned embryos produced by oocyte bisection cloning.PloS one · 2015Article
- Enhancing effects of serum-rich and cytokine-supplemented culture conditions on developing blastocysts and deriving porcine parthenogenetic embryonic stem cells.Journal of veterinary science · 2014Article
- Reprogramming of pig dermal fibroblast into insulin secreting cells by a brief exposure to 5-aza-cytidine.Stem cell reviews and reports · 2014Article
- From "ES-like" cells to induced pluripotent stem cells: a historical perspective in domestic animals.Theriogenology · 2014Review
- Generation of minipigs with targeted transgene insertion by recombinase-mediated cassette exchange (RMCE) and somatic cell nuclear transfer (SCNT).Transgenic research · 2013Article
- Stem cell potency and the ability to contribute to chimeric organisms.Reproduction (Cambridge, England) · 2013Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The generation of porcine embryonic stem cells (pESC) would potentially have great impact in the biomedical field given the long-standing history of the pig as a prime animal model for pre-clinical biomedical applications. These cells would also be beneficial for the agricultural area, allowing efficient genetic engineering of this animal, to improve health and production traits. Despite numerous reports, no conclusive results have been obtained on the isolation and propagation of pESC lines and the establishment of pluripotent cells from the pig has remained an elusive goal. In the present study we performed a systematic analysis of different culture media for their ability to support the establishment of homogenous outgrowths from in vitro-produced embryos. Furthermore, we investigated which molecular networks are responsive to the factors contained in the most efficient media, since the identification of dominant signaling pathways that regulate porcine stem-cell pluripotency is likely to facilitate the generation of genuine pESC. Finally we compared IVF blastocysts versus parthenotes as a possible source for putative pESC in terms of blastocyst rate, resilience to immunosurgery procedures, ability to attach to the feeder, to generate outgrowths and to establish stable cell lines.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.