ArticleThe Journal of clinical investigation1991
Shape changes, exocytosis, and cytosolic free calcium changes in stimulated human eosinophils.
Article in The Journal of clinical investigation, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 114 citations in OpenAlex.
- A phase 2 study to evaluate the safety, efficacy and pharmacokinetics of DP2 antagonist GB001 and to explore biomarkers of airway inflammation in mild-to-moderate asthma.Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology · 2020Trial
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- DisruptingMicroorganisms · 2020Article
- Alternaria fungus induces the production of GM-CSF, interleukin-6 and interleukin-8 and calcium signaling in human airway epithelium through protease-activated receptor 2.International archives of allergy and immunology · 2012Article
- Eosinophils: multifaceted biological properties and roles in health and disease.Immunological reviews · 2011Review
- Recognition of fungal protease activities induces cellular activation and eosinophil-derived neurotoxin release in human eosinophils.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
- Protein kinase C activation inhibits eosinophil degranulation through stimulation of intracellular cAMP production.British journal of pharmacology · 2004Article
- A parallel signal-transduction pathway for eotaxin- and interleukin-5-induced eosinophil shape change.Immunology · 2003Article
- A role for humoral mechanisms in the pathogenesis of Devic's neuromyelitis optica.Brain : a journal of neurology · 2002Article
- Cytokines in asthma.Thorax · 1999Review
- Adenosine A3 receptors on human eosinophils mediate inhibition of degranulation and superoxide anion release.British journal of pharmacology · 1999Article
- Transcriptional activation of the interleukin-8 gene by respiratory syncytial virus infection in alveolar epithelial cells: nuclear translocation of the RelA transcription factor as a mechanism producing airway mucosal inflammation.Journal of virology · 1996Article
- Voltage-activated proton current in eosinophils from human blood.The Journal of physiology · 1996Article
- Expression of human platelet-activating factor receptor gene in EoL-1 cells following butyrate-induced differentiation.The Biochemical journal · 1995Article
- Binding to heparan sulfate or heparin enhances neutrophil responses to interleukin 8.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- Article
- Cloning and functional expression of the canine anaphylatoxin C5a receptor. Evidence for high interspecies variability.The Biochemical journal · 1992Article
- RANTES and macrophage inflammatory protein 1 alpha induce the migration and activation of normal human eosinophil granulocytes.The Journal of experimental medicine · 1992Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Essentially pure preparations of normal density eosinophils obtained from patients with hypereosinophilic syndrome (HES) were stimulated with complement factor 5a (C5a), platelet-activating factor (PAF), FMLP and neutrophil-activating peptide (NAP-1/IL-8). Three responses were studied, the transient rise in cytosolic free calcium concentration ([Ca2+]i) (derived from indo-1 fluorescence), shape changes (measured by laser turbidimetry), and exocytosis of eosinophil peroxidase (EPO) (assessed by H2O2/luminol-dependent chemiluminescence). Responses were obtained with all four agonists, but C5a and PAF were by far more potent than FMLP and NAP-1/IL-8, which induced only minor effects. Pretreatment of the cells with pertussis toxin attenuated [Ca2+]i changes, EPO release and, to a lesser extent, shape changes, indicating that GTP-binding proteins of Gi-type are involved in receptor-dependent signal transduction processes leading to these responses. A clear dissociation was observed in the control of the shape change response and EPO exocytosis. The shape change was not affected by Ca2+ depletion or treatment with the protein kinase inhibitor staurosporine, but exocytosis was prevented by Ca2+ depletion and markedly enhanced by staurosporine. The activation of the contractile system, leading to shape changes and motility, thus appears to be independent of the classical signal transduction pathway involving phospholipase C, a [Ca2+]i rise and protein kinase C activation. Exocytosis is, as expected, Ca2+ dependent and appears to be under a negative control involving protein phosphorylations.
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