ArticleThe Journal of biological chemistry2010
GABAB receptor constituents revealed by tandem affinity purification from transgenic mice.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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Who cites it
25 citing papers in PubMed, 48 citations in OpenAlex.
- The G protein modifier KCTD5 tunes the decoding of neuromodulatory signals necessary for motor function in striatal neurons.PLoS biology · 2025Article
- KCTD8 and KCTD12 Facilitate Axonal Expression of GABAThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Article
- GABACurrent topics in behavioral neurosciences · 2022Review
- A Picture Worth a Thousand Molecules-Integrative Technologies for Mapping Subcellular Molecular Organization and Plasticity in Developing Circuits.Frontiers in synaptic neuroscience · 2020Article
- Structural basis for auxiliary subunit KCTD16 regulation of the GABAProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Structural biology of GABANeuropharmacology · 2018Review
- Diversity of structure and function of GABAProceedings of the Japan Academy. Series B, Physical and biological sciences · 2018Review
- KCTD12 modulation of GABA(B) receptor function.Pharmacology research & perspectives · 2017Article
- Epilepsy and intellectual disability linked protein Shrm4 interaction with GABANature communications · 2017Article
- KCTD Hetero-oligomers Confer Unique Kinetic Properties on Hippocampal GABAB Receptor-Induced K+ Currents.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- In Vivo Interaction Proteomics in Caenorhabditis elegans Embryos Provides New Insights into P Granule Dynamics.Molecular & cellular proteomics : MCP · 2016Article
- KCTD12 Regulates Colorectal Cancer Cell Stemness through the ERK Pathway.Scientific reports · 2016Article
- Quantitative affinity purification mass spectrometry: a versatile technology to study protein-protein interactions.Frontiers in genetics · 2015Review
- Article
- Complex GABAB receptor complexes: how to generate multiple functionally distinct units from a single receptor.Frontiers in pharmacology · 2014Review
- Article
- The Concise Guide to PHARMACOLOGY 2013/14: G protein-coupled receptors.British journal of pharmacology · 2013Article
- Up-regulation of GABA(B) receptor signaling by constitutive assembly with the K+ channel tetramerization domain-containing protein 12 (KCTD12).The Journal of biological chemistry · 2013Article
- Novel, gel-free proteomics approach identifies RNF5 and JAMP as modulators of GPCR stability.Molecular endocrinology (Baltimore, Md.) · 2013Article
- Opposite effects of KCTD subunit domains on GABA(B) receptor-mediated desensitization.The Journal of biological chemistry · 2012Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
GABA(B) receptors function as heterodimeric G-protein-coupled receptors for the neurotransmitter gamma-aminobutyric acid (GABA). Receptor subtypes, based on isoforms of the ligand-binding subunit GABA(B1), are thought to involve a differential set of associated proteins. Here, we describe two mouse lines that allow a straightforward biochemical isolation of GABA(B) receptors. The transgenic mice express GABA(B1) isoforms that contain sequences for a two-step affinity purification, in addition to their endogenous subunit repertoire. Comparative analyses of purified samples from the transgenic mice and wild-type control animals revealed two novel components of the GABA(B1) complex. One of the identified proteins, potassium channel tetramerization domain-containing protein 12, associates with heterodimeric GABA(B) receptors via the GABA(B2) subunit. In transfected hippocampal neurons, potassium channel tetramerization domain-containing protein 12 augmented axonal surface targeting of GABA(B2). The mice equipped with tags on GABA(B1) facilitate validation and identification of native binding partners of GABA(B) receptors, providing insight into the molecular mechanisms of synaptic modulation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.