ArticleEnvironmental microbiology2010
Polyamines are required for the expression of key Hms proteins important for Yersinia pestis biofilm formation.
Article in Environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed, 60 citations in OpenAlex.
- The Homoserine DehydrogenaseMicroorganisms · 2026Article
- Research progress on the microbial metabolism and transport of polyamines and their roles in animal gut homeostasis.Journal of animal science and biotechnology · 2025Review
- A bibliography study of Shewanella oneidensis biofilm.FEMS microbiology ecology · 2023Article
- Dickeya Manipulates Multiple Quorum Sensing Systems to Control Virulence and Collective Behaviors.Frontiers in plant science · 2022Review
- Effect of Spermidine on Biofilm Formation in Escherichia coli K-12.Journal of bacteriology · 2021Article
- Genome-scale metabolic reconstruction and in silico analysis of the rice leaf blight pathogen, Xanthomonas oryzae.Molecular plant pathology · 2020Article
- Prediction of Burkholderia pseudomallei DsbA substrates identifies potential virulence factors and vaccine targets.PloS one · 2020Article
- Microbial Metabolites Determine Host Health and the Status of Some Diseases.International journal of molecular sciences · 2019Review
- Metabolomics Deciphered Metabolic Reprogramming Required for Biofilm Formation.Scientific reports · 2019Article
- Putrescine Is an Intraspecies and Interkingdom Cell-Cell Communication Signal Modulating the Virulence ofFrontiers in microbiology · 2019Article
- Polyamine function in archaea and bacteria.The Journal of biological chemistry · 2018Review
- Pulmonary Surfactant Promotes Virulence Gene Expression and Biofilm Formation in Klebsiella pneumoniae.Infection and immunity · 2018Article
- The Role of Cadaverine Synthesis on Pneumococcal Capsule and Protein Expression.Medical sciences (Basel, Switzerland) · 2018Article
- Article
- The virulence-associated protein HsvA from the fire blight pathogenThe Journal of biological chemistry · 2017Article
- Spermidine promotesThe Journal of biological chemistry · 2017Article
- Transcriptomic Analysis of the Adaptation ofFrontiers in microbiology · 2017Article
- Activity of Norspermidine on Bacterial Biofilms of Multidrug-Resistant Clinical Isolates Associated with Persistent Extremity Wound Infections.Advances in experimental medicine and biology · 2017Article
- Norspermidine changes the basic structure of S. mutans biofilm.Molecular medicine reports · 2017Article
- Substrate specificity and function of acetylpolyamine amidohydrolases from Pseudomonas aeruginosa.BMC biochemistry · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We previously showed that mutations in the genes encoding the two main biosynthetic enzymes responsible for polyamine production, arginine decarboxylase (SpeA) and ornithine decarboxylase (SpeC) cause a loss of biofilm formation in Yersinia pestis. In Y. pestis the development of a biofilm is dependent on 6 Hms (hemin storage) proteins (HmsH, F, R, S, T and P) grouped into 3 operons; hmsHFRS, hmsT and hmsP. In this article we show that polyamines are necessary to maintain the levels of key Hms proteins. In the absence of polyamines there is an approximately 93%, approximately 43% and approximately 90% reduction in protein levels of HmsR, HmsS and HmsT respectively. Overexpression of hmsR and hmsT from plasmids alone can restore biofilm formation to a SpeA(-)SpeC(-) mutant. Addition of exogenous putrescine also restores normal levels of HmsR, HmsS, HmsT and biofilm production. Analyses using transcriptional reporters and quantitative RT-PCR indicate that the initiation of transcription and mRNA stability are not reduced by polyamine deficiency. Instead, translational reporters indicate that polyamines function at least in part by modulating the translation of HmsR and HmsT. Although construction of a consensus Shine-Dalgarno sequence upstream of hmsT modestly reduced the stimulation of translation by putrescine, additional mechanisms likely contribute to the polyamine-dependent expression of HmsT. Finally, we have shown that polyamines play a role in bubonic plague.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.