ArticleEnvironmental microbiology2010
Microaerobic conditions enhance type III secretion and adherence of enterohaemorrhagic Escherichia coli to polarized human intestinal epithelial cells.
Article in Environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 53 citations in OpenAlex.
- Article
- Isolation of a novel Lactiplantibacillus plantarum strain resistant to nitrite stress and its transcriptome analysis.Journal of microbiology (Seoul, Korea) · 2022Article
- Development of a novel human intestinal model to elucidate the effect of anaerobic commensals on Escherichia coli infection.Disease models & mechanisms · 2022Article
- Pathogenicity and virulence regulation ofVirulence · 2020Review
- Enterohaemorrhagic Escherichia coli activates nitrate respiration to benefit from the inflammatory response for initiation of microcolony-formation.BMC microbiology · 2020Article
- Recent Progress on the Electrochemical Biosensing ofBiosensors · 2020Review
- The sRNA DicF integrates oxygen sensing to enhance enterohemorrhagicProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Oxygen and contact with human intestinal epithelium independently stimulate virulence gene expression in enteroaggregative Escherichia coli.Cellular microbiology · 2019Article
- ProbingFrontiers in microbiology · 2019Article
- Modulation of EnterohaemorrhagicMicroorganisms · 2018Review
- Pathogens' adaptation to the human host.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Shiga toxin 2 translocation across intestinal epithelium is linked to virulence of Shiga toxin-producing Escherichia coli in humans.Microbiology (Reading, England) · 2018Article
- A multi-omic analysis reveals the role of fumarate in regulating the virulence of enterohemorrhagic Escherichia coli.Cell death & disease · 2018Article
- Enterohemorrhagic Escherichia coli outwits hosts through sensing small molecules.Current opinion in microbiology · 2018Review
- Food-Associated Stress Primes Foodborne Pathogens for the Gastrointestinal Phase of Infection.Frontiers in microbiology · 2018Review
- Emulating Host-Microbiome Ecosystem of Human Gastrointestinal Tract in Vitro.Stem cell reviews and reports · 2017Review
- Article
- The Two-Component System CpxRA Negatively Regulates the Locus of Enterocyte Effacement of Enterohemorrhagic Escherichia coli Involving σ(32) and Lon protease.Frontiers in cellular and infection microbiology · 2016Article
- Type III secretion systems: the bacterial flagellum and the injectisome.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2015Review
- Enterohemorrhagic Escherichia coli colonization of human colonic epithelium in vitro and ex vivo.Infection and immunity · 2015Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Advances in the understanding of the pathogenesis of enterohaemorrhagic Escherichia coli (EHEC) have greatly benefited from the use of human epithelial cell lines under aerobic conditions. However, in the target site of EHEC infection, the human intestine, conditions are microaerobic. In our study we used polarized human colon carcinoma cells in a vertical diffusion chamber system to investigate the influence of reduced apical oxygen levels on EHEC colonization. While apical microaerobiosis did not affect cell integrity and barrier function, numbers of adherent bacteria were significantly increased under low compared with high apical oxygen concentrations. In addition, expression and translocation of EHEC type III secreted (T3S) effector proteins was considerably enhanced under microaerobic conditions and dependent on the presence of host cells. Increased colonization was mainly mediated via EspA as adherence levels of an isogenic deletion mutant were not influenced by low oxygen levels. Other potential adherence factors (E. coli common pilus and flagella) were only minimally expressed under high and low oxygen levels. Addition of nitrate and trimethylamine N-oxide as terminal electron acceptors for anaerobic respiration failed to further increase bacterial colonization or T3S under microaerobiosis. This study indicates that EHEC T3S and colonization are enhanced by the microaerobic environment in the gut and therefore might be underestimated in conventional aerobic cell culture systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.