ArticleDiabetologia2010
In vivo non-viral gene delivery of human vascular endothelial growth factor improves revascularisation and restoration of euglycaemia after human islet transplantation into mouse liver.
Article in Diabetologia, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 57 citations in OpenAlex.
- β-cell-targeted RNA activation of vascular endothelial growth factor-A improves islet transplantation.Signal transduction and targeted therapy · 2026Article
- Review
- Near-infrared light-responsive hydrogels for on-demand dual delivery of proangiogenic growth factors.Acta biomaterialia · 2024Article
- Sustained heterologous gene expression in pancreatic islet organoids using adeno-associated virus serotype 8.Frontiers in bioengineering and biotechnology · 2023Article
- Delivery of therapeutic agents and cells to pancreatic islets: Towards a new era in the treatment of diabetes.Molecular aspects of medicine · 2022Review
- Recent ultrasound advancements for the manipulation of nanobiomaterials and nanoformulations for drug delivery.Ultrasonics sonochemistry · 2021Review
- Islet cell encapsulation - Application in diabetes treatment.Experimental biology and medicine (Maywood, N.J.) · 2021Review
- Fibroblast growth factor 7 releasing particles enhance islet engraftment and improve metabolic control following islet transplantation in mice with diabetes.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2021Article
- Novel Coagulation Factor VIII Gene Therapy in a Mouse Model of Hemophilia A by Lipid-Coated FeBiomedicines · 2021Article
- The promising shadow of microbubble over medical sciences: from fighting wide scope of prevalence disease to cancer eradication.Journal of biomedical science · 2021Review
- Oxygen and nutrient delivery in tissue engineering: Approaches to graft vascularization.Journal of tissue engineering and regenerative medicine · 2019Review
- β-Cell Receptor Tyrosine Kinases in Controlling Insulin Secretion and Exocytotic Machinery: c-Kit and Insulin Receptor.Endocrinology · 2018Review
- The Role of Accessory Cells in Islet Homeostasis.Current diabetes reports · 2018Review
- Article
- Localized Delivery of shRNA against PHD2 Protects the Heart from Acute Myocardial Infarction through Ultrasound-Targeted Cationic Microbubble Destruction.Theranostics · 2017Article
- ANGPTL8 reverses established adriamycin cardiomyopathy by stimulating adult cardiac progenitor cells.Oncotarget · 2016Article
- Scaffold-mediated BMP-2 minicircle DNA delivery accelerated bone repair in a mouse critical-size calvarial defect model.Journal of biomedical materials research. Part A · 2016Article
- Transient Suppression of TGFβ Receptor Signaling Facilitates Human Islet Transplantation.Endocrinology · 2016Article
- Article
- Engineered VEGF-releasing PEG-MAL hydrogel for pancreatic islet vascularization.Drug delivery and translational research · 2015Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
aims/hypothesisDelivery of the gene for human vascular endothelial growth factor (VEGF, also known as VEGFA) to both the transplanted islets and the surrounding tissue may promote islet revascularisation and survival. We previously showed the effective delivery of VEGF gene to rat myocardium by an ultrasound-mediated gene-transfer method named ultrasound-targeted microbubble destruction (UTMD). Here we examined the effect of non-viral VEGF delivery using UTMD on transplanted islets in vivo.
methodsA marginal number of human islets were transplanted into livers of mice which were a model for diabetes. Then, non-viral plasmid vectors encoding VEGF (VEGF group, n = 11) or the gene for green fluorescent protein (GFP) (GFP group, n = 7) were introduced into the host liver by UTMD. Transplantation without gene delivery was performed as a control (no-UTMD group, n = 8). Blood glucose, serum human insulin, C-peptide levels and the revascularisation in graft islets were evaluated.
resultsRestoration of euglycaemia occurred in 13% in the no-UTMD group and 14% in the GFP group, whereas 73% mice in the VEGF group became euglycaemic at day 30 (p < 0.05 in no-UTMD vs VEGF). Serum human insulin and C-peptide were significantly higher in the VEGF group at day 32 (insulin: no-UTMD, 17 +/- 8; GFP, 37 +/- 17; VEGF, 109 +/- 26 pmol/l, respectively, p < 0.05; C-peptide: no-UTMD, 68 +/- 38; GFP, 115 +/- 58; VEGF, 791 +/- 230 pmol/l, respectively, p < 0.05). Vessel density in graft islets was significantly higher in the VEGF group (no-UTMD, 169 +/- 36; GFP, 227 +/- 39; VEGF, 649 +/- 51 counts/mm(2), respectively, p < 0.05). CONCLUSIONS/
interpretationDelivery of VEGF gene to host liver using UTMD promoted islet revascularisation after islet transplantation and improved the restoration of euglycaemia.
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