ArticleRNA biology
Enhanced purity, activity and structural integrity of yeast ribosomes purified using a general chromatographic method.
Article in RNA biology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 19 citations in OpenAlex.
- Use of Baby Spinach and Broccoli for imaging of structured cellular RNAs.Nucleic acids research · 2017Article
- Article
- Structure of the ribosome post-recycling complex probed by chemical cross-linking and mass spectrometry.Nature communications · 2016Article
- Eukaryotic rpL10 drives ribosomal rotation.Nucleic acids research · 2014Article
- Proofreading of pre-40S ribosome maturation by a translation initiation factor and 60S subunits.Nature structural & molecular biology · 2012Article
- rRNA pseudouridylation defects affect ribosomal ligand binding and translational fidelity from yeast to human cells.Molecular cell · 2011Article
- Chromatographic purification of highly active yeast ribosomes.Journal of visualized experiments : JoVE · 2011Article
- A flexible loop in yeast ribosomal protein L11 coordinates P-site tRNA binding.Nucleic acids research · 2010Article
- A molecular clamp ensures allosteric coordination of peptidyltransfer and ligand binding to the ribosomal A-site.Nucleic acids research · 2010Article
- Article
- Mass Spectrometry Analysis of Nucleic Acid Modifications: From Beginning to Future.Mass spectrometry reviewsReview
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Authors and funding
4 authors at 2 institutions in 4 countries.
Funding
Abstract
One of the major challenges facing researchers working with eukaryotic ribosomes lies in their lability relative to their eubacterial and archael counterparts. In particular, lysis of cells and purification of eukaryotic ribosomes by conventional differential ultracentrifugation methods exposes them for long periods of time to a wide range of co-purifying proteases and nucleases, negatively impacting their structural integrity and functionality. A chromatographic method using a cysteine charged Sulfolink resin was adapted to address these problems. This fast and simple method significantly reduces co-purifying proteolytic and nucleolytic activities, producing good yields of highly biochemically active yeast ribosomes with fewer nicks in their rRNAs. In particular, the chromatographic purification protocol significantly improved the quality of ribosomes isolated from mutant cells. This method is likely applicable to mammalian ribosomes as well. The simplicity of the method, and the enhanced purity and activity of chromatographically purified ribosome represents a significant technical advancement for the study of eukaryotic ribosomes.
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