ArticleProceedings of the National Academy of Sciences of the United States of America2010
Helper-independent piggyBac plasmids for gene delivery approaches: strategies for avoiding potential genotoxic effects.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- Highly efficient and low-mosaicismiScience · 2026Article
- Inducible trophoblast-specific knockdown of mechanistic target of rapamycin impairs placental folate transport in mice.The Journal of nutritional biochemistry · 2026Article
- PiggyBac transposase-mediated inducible trophoblast-specific knockdown of Mtor decreases placental nutrient transport and fetal growth.Clinical science (London, England : 1979) · 2025Article
- Trophoblast-specific Deptor knockdown enhances trophoblast nutrient transport and fetal growth in mice.Acta physiologica (Oxford, England) · 2025Article
- Contributions of Ryuzo Yanagimachi to the field of reproductive biology.Biology of reproduction · 2019Article
- Transposase-mediated gene modulation in the placenta.Placenta · 2017Review
- Use of mariner transposases for one-step delivery and integration of DNA in prokaryotes and eukaryotes by transfection.Nucleic acids research · 2017Article
- Pyrophosphate Supplementation Prevents Chronic and Acute Calcification in ABCC6-Deficient Mice.The American journal of pathology · 2017Article
- Enhancing the genome editing toolbox: genome wide CRISPR arrayed libraries.Scientific reports · 2017Article
- Tβ4-overexpression based on the piggyBac transposon system in cashmere goats alters hair fiber characteristics.Transgenic research · 2017Article
- Production of functional human nerve growth factor from the saliva of transgenic mice by using salivary glands as bioreactors.Scientific reports · 2017Article
- The Functionality of Minimal PiggyBac Transposons in Mammalian Cells.Molecular therapy. Nucleic acids · 2016Article
- Characterization of Growth and Reproduction Performance, Transgene Integration, Expression, and Transmission Patterns in Transgenic Pigs Produced by piggyBac Transposition-Mediated Gene Transfer.Animal biotechnology · 2016Article
- Ultrasound-targeted hepatic delivery of factor IX in hemophiliac mice.Gene therapy · 2016Article
- Induced pluripotent stem cells in Alzheimer's disease: applications for disease modeling and cell-replacement therapy.Molecular neurodegeneration · 2016Review
- Evaluating the potential for undesired genomic effects of the piggyBac transposon system in human cells.Nucleic acids research · 2015Article
- Adjuvants may reduce in vivo transfection levels for DNA vaccination in mice leading to reduced antigen-specific CD8+ T cell responses.Human vaccines & immunotherapeutics · 2015Article
- A hyperactive piggyBac transposon system is an easy-to-implement method for introducing foreign genes into mouse preimplantation embryos.The Journal of reproduction and development · 2015Article
- Vector modifications to eliminate transposase expression following piggyBac-mediated transgenesis.Scientific reports · 2014Article
- Medical school hotline: The Institute for Biogenesis Research: a flower in the Pacific.Hawai'i journal of medicine & public health : a journal of Asia Pacific Medicine & Public Health · 2014Article
Corrections and comments
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Authors and funding
14 authors.
Funding
Abstract
Efficient integration of functional genes is an essential prerequisite for successful gene delivery such as cell transfection, animal transgenesis, and gene therapy. Gene delivery strategies based on viral vectors are currently the most efficient. However, limited cargo capacity, host immune response, and the risk of insertional mutagenesis are limiting factors and of concern. Recently, several groups have used transposon-based approaches to deliver genes to a variety of cells. The piggyBac (pB) transposase in particular has been shown to be well suited for cell transfection and gene therapy approaches because of its flexibility for molecular modification, large cargo capacity, and high transposition activity. However, safety considerations regarding transposase gene insertions into host genomes have rarely been addressed. Here we report our results on engineering helper-independent pB plasmids. The single-plasmid gene delivery system carries both the piggyBac transposase (pBt) expression cassette as well as the transposon cargo flanked by terminal repeat element sequences. Improvements to the helper-independent structure were achieved by developing new plasmids in which the pBt gene is rendered inactive after excision of the transposon from the plasmid. As a consequence, potentially negative effects that may develop by the persistence of an active pBt gene posttransposition are eliminated. The results presented herein demonstrate that our helper-independent plasmids represent an important step in the development of safe and efficient gene delivery methods that should prove valuable in gene therapy and transgenic approaches.
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