ArticleProceedings of the National Academy of Sciences of the United States of America2010
H-NOX-mediated nitric oxide sensing modulates symbiotic colonization by Vibrio fischeri.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed, 110 citations in OpenAlex.
- A single point mutation is sufficient to driveJournal of bacteriology · 2025Article
- Oxidative Activation of the Heme Nitric Oxide/Oxygen-Binding Protein (H-NOX) fromBiochemistry · 2025Article
- Host-Associated Biofilms:Microorganisms · 2025Review
- Lighting the way: how theJournal of bacteriology · 2024Review
- Review
- Flagella are required to coordinately activate competition and host colonization factors in response to a mechanical signal.bioRxiv : the preprint server for biology · 2024Article
- The H-NOX protein structure adapts to different mechanisms in sensors interacting with nitric oxide.Chemical science · 2023Article
- Host hydrocarbons protect symbiont transmission from a radical host defense.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Genetic Analysis Reveals a Requirement for the Hybrid Sensor Kinase RscS inJournal of bacteriology · 2023Article
- Gas and light: triggers of c-di-GMP-mediated regulation.FEMS microbiology reviews · 2023Review
- H-NOX Regulates Biofilm Formation inBiochemistry · 2023Article
- Using axenic and gnotobiotic insects to examine the role of different microbes on the development and reproduction of the kissing bug Rhodnius prolixus (Hemiptera: Reduviidae).Molecular ecology · 2023Article
- Quantification of the capacity of vibrio fischeri to establish symbiosis with Euprymna scolopes.PloS one · 2023Article
- How It All Begins: Bacterial Factors Mediating the Colonization of Invertebrate Hosts by Beneficial Symbionts.Microbiology and molecular biology reviews : MMBR · 2022Review
- The Alginate and Motility Regulator AmrZ is Essential for the Regulation of the Dispersion Response bymSphere · 2022Article
- Nitric Oxide, Nitric Oxide Formers and Their Physiological Impacts in Bacteria.International journal of molecular sciences · 2022Review
- H-NOX proteins in the virulence of pathogenic bacteria.Bioscience reports · 2022Review
- Three Microbial Musketeers of the Seas:International journal of molecular sciences · 2022Article
- Polyunsaturated fatty acids cause physiological and behavioral changes in Vibrio alginolyticus and Vibrio fischeri.MicrobiologyOpen · 2021Article
- A lasting symbiosis: how Vibrio fischeri finds a squid partner and persists within its natural host.Nature reviews. Microbiology · 2021Review
3 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The bioluminescent bacterium Vibrio fischeri initiates a specific, persistent symbiosis in the light organ of the squid Euprymna scolopes. During the early stages of colonization, V. fischeri is exposed to host-derived nitric oxide (NO). Although NO can be both an antimicrobial component of innate immunity and a key signaling molecule in eukaryotes, potential roles in beneficial host-microbe associations have not been described. V. fischeri hnoX encodes a heme NO/oxygen-binding (H-NOX) protein, a member of a family of bacterial NO- and/or O(2)-binding proteins of unknown function. We hypothesized that H-NOX acts as a NO sensor that is involved in regulating symbiosis-related genes early in colonization. Whole-genome expression studies identified 20 genes that were repressed in an NO- and H-NOX-dependent fashion. Ten of these, including hemin-utilization genes, have a promoter with a putative ferric-uptake regulator (Fur) binding site. As predicted, in the presence of NO, wild-type V. fischeri grew more slowly on hemin than a hnoX deletion mutant. Host-colonization studies showed that the hnoX mutant was also 10-fold more efficient in initially colonizing the squid host than the wild type; similarly, in mixed inoculations, it outcompeted the wild-type strain by an average of 16-fold after 24 h. However, the presence of excess hemin or iron reversed this dominance. The advantage of the mutant in colonizing the iron-limited light-organ tissues is caused, at least in part, by its greater ability to acquire host-derived hemin. Our data suggest that V. fischeri normally senses a host-generated NO signal through H-NOX(Vf) and modulates the expression of its iron uptake capacity during the early stages of the light-organ symbiosis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.