ArticleThe Journal of cell biology2010
HPat provides a link between deadenylation and decapping in metazoa.
Article in The Journal of cell biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed.
- Greatwall-Endos-PP2A/B55Open biology · 2024Article
- Protein interaction network revealed by quantitative proteomic analysis links TFIIB to multiple aspects of the transcription cycle.Biochimica et biophysica acta. Proteins and proteomics · 2024Article
- Eukaryotic mRNA decapping factors: molecular mechanisms and activity.The FEBS journal · 2023Review
- Plasticity of Drosophila germ granules during germ cell development.PLoS biology · 2023Article
- Structure and function of molecular machines involved in deadenylation-dependent 5'-3' mRNA degradation.Frontiers in genetics · 2023Review
- Eukaryotic mRNA Decapping Activation.Frontiers in genetics · 2022Review
- Unique repression domains of Pumilio utilize deadenylation and decapping factors to accelerate destruction of target mRNAs.Nucleic acids research · 2020Article
- The Dynamics of Cytoplasmic mRNA Metabolism.Molecular cell · 2020Article
- MicroRNAs Cause Accelerated Decay of Short-Tailed Target mRNAs.Molecular cell · 2020Article
- Pat1 activates late steps in mRNA decay by multiple mechanisms.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Direct role for the Drosophila GIGYF protein in 4EHP-mediated mRNA repression.Nucleic acids research · 2019Article
- Mechanistic Insights into MicroRNA-Mediated Gene Silencing.Cold Spring Harbor perspectives in biology · 2019Review
- Inhibiting transcription in cultured metazoan cells with actinomycin D to monitor mRNA turnover.Methods (San Diego, Calif.) · 2019Review
- mRNA decapping: finding the right structures.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Review
- Polysomes, Stress Granules, and Processing Bodies: A Dynamic Triumvirate Controlling Cytoplasmic mRNA Fate and Function.Plant physiology · 2018Review
- A unique surface on Pat1 C-terminal domain directly interacts with Dcp2 decapping enzyme and Xrn1 5'-3' mRNA exonuclease in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Female Infertility Caused by Mutations in the Oocyte-Specific Translational Repressor PATL2.American journal of human genetics · 2017Article
- Translational repression of theRNA (New York, N.Y.) · 2017Article
- Article
- Dual RNA Processing Roles of Pat1b via Cytoplasmic Lsm1-7 and Nuclear Lsm2-8 Complexes.Cell reports · 2017Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Decapping of eukaryotic messenger RNAs (mRNAs) occurs after they have undergone deadenylation, but how these processes are coordinated is poorly understood. In this study, we report that Drosophila melanogaster HPat (homologue of Pat1), a conserved decapping activator, interacts with additional decapping factors (e.g., Me31B, the LSm1-7 complex, and the decapping enzyme DCP2) and with components of the CCR4-NOT deadenylase complex. Accordingly, HPat triggers deadenylation and decapping when artificially tethered to an mRNA reporter. These activities reside, unexpectedly, in a proline-rich region. However, this region alone cannot restore decapping in cells depleted of endogenous HPat but also requires the middle (Mid) and the very C-terminal domains of HPat. We further show that the Mid and C-terminal domains mediate HPat recruitment to target mRNAs. Our results reveal an unprecedented role for the proline-rich region and the C-terminal domain of metazoan HPat in mRNA decapping and suggest that HPat is a component of the cellular mechanism that couples decapping to deadenylation in vivo.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.