ArticleExperimental biology and medicine (Maywood, N.J.)2010
Insulin inhibits human erythrocyte cAMP accumulation and ATP release: role of phosphodiesterase 3 and phosphoinositide 3-kinase.
Article in Experimental biology and medicine (Maywood, N.J.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 38 citations in OpenAlex.
- The Product of Red Blood Cells and Hematocrit Can Be Used as a Novel Indicator of Impaired Fasting Blood Glucose Status.Diabetes, metabolic syndrome and obesity : targets and therapy · 2020Article
- Reduced deformability contributes to impaired deoxygenation-induced ATP release from red blood cells of older adult humans.The Journal of physiology · 2019Article
- Leptin Stimulates Cellular Glycolysis Through a STAT3 Dependent Mechanism in Tilapia.Frontiers in endocrinology · 2018Article
- Purinergic control of red blood cell metabolism: novel strategies to improve red cell storage quality.Blood transfusion = Trasfusione del sangue · 2017Review
- Akt/mTOR Role in Human Foetoplacental Vascular Insulin Resistance in Diseases of Pregnancy.Journal of diabetes research · 2017Review
- Role of erythrocyte-released ATP in the regulation of microvascular oxygen supply in skeletal muscle.Acta physiologica (Oxford, England) · 2016Review
- Blood cells: an historical account of the roles of purinergic signalling.Purinergic signalling · 2015Review
- Liposomal delivery of a phosphodiesterase 3 inhibitor rescues low oxygen-induced ATP release from erythrocytes of humans with type 2 diabetes.Biochemistry and biophysics reports · 2015Article
- Lymphatic vascular integrity is disrupted in type 2 diabetes due to impaired nitric oxide signalling.Cardiovascular research · 2015Article
- Regulation of extracellular ATP in human erythrocytes infected with Plasmodium falciparum.PloS one · 2014Article
- Synergistic effects of C-peptide and insulin on low O2-induced ATP release from human erythrocytes.American journal of physiology. Regulatory, integrative and comparative physiology · 2013Article
- Kinetics of extracellular ATP in mastoparan 7-activated human erythrocytes.Biochimica et biophysica acta · 2013Article
- Cyclic phosphatidic acid stimulates cAMP production and inhibits growth in human colon cancer cells.PloS one · 2013Article
- Erythrocyte-derived ATP and perfusion distribution: role of intracellular and intercellular communication.Microcirculation (New York, N.Y. : 1994) · 2012Review
- Cyclic nucleotide phosphodiesterase (PDE) isozymes as targets of the intracellular signalling network: benefits of PDE inhibitors in various diseases and perspectives for future therapeutic developments.British journal of pharmacology · 2012Review
- Toward a multiscale description of microvascular flow regulation: o(2)-dependent release of ATP from human erythrocytes and the distribution of ATP in capillary networks.Frontiers in physiology · 2012Article
- A selective phosphodiesterase 3 inhibitor rescues low PO2-induced ATP release from erythrocytes of humans with type 2 diabetes: implication for vascular control.American journal of physiology. Heart and circulatory physiology · 2011Article
- Erythrocytes as controllers of perfusion distribution in the microvasculature of skeletal muscle.Acta physiologica (Oxford, England) · 2011Review
- Defects in oxygen supply to skeletal muscle of prediabetic ZDF rats.American journal of physiology. Heart and circulatory physiology · 2010Article
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Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
In non-erythroid cells, insulin stimulates a signal transduction pathway that results in the activation of phosphoinositide 3-kinase (PI3K) and subsequent phosphorylation of phosphodiesterase 3 (PDE3). Erythrocytes possess insulin receptors, PI3K and PDE3B. These cells release adenosine triphosphate (ATP) when exposed to reduced O(2) tension via a signaling pathway that requires activation of the G protein, Gi, as well as increases in cAMP. Although insulin inhibits ATP release from human erythrocytes in response to Gi activation by mastoparan 7 (Mas 7), no effect on cAMP was described. Here, we investigated the hypothesis that insulin activates PDE3 in human erythrocytes via a PI3K-mediated mechanism resulting in cAMP hydrolysis and inhibition of ATP release. Incubation of human erythrocytes with Mas 7 resulted in a 62 +/- 7% increase in cAMP (n = 9, P < 0.05) and a 306 +/- 69% increase in ATP release (n = 9, P < 0.05), both of which were attenuated by pre-treatment with insulin. Selective inhibitors of PDE3 (cilostazol) or PI3K (LY294002) rescued these effects of insulin. These results support the hypothesis that insulin activates PDE3 in erythrocytes via a PI3K-dependent mechanism. Once activated, PDE3 limits Mas 7-induced increases in intracellular cAMP. This effect of insulin leads, ultimately, to decreased ATP release in response to Mas 7. Activation of Gi is required for reduced O(2) tension-induced ATP release from erythrocytes and this ATP release has been shown to participate in the matching of O(2) supply with demand in skeletal muscle. Thus, pathological increases in circulating insulin could, via activation of PDE3 in erythrocytes, inhibit ATP release from these cells, depriving the peripheral circulation of one mechanism that could aid in the regulation of the delivery of O(2) to meet tissue metabolic need.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.