ArticleVirology journal2010
Influenza A viral loads in respiratory samples collected from patients infected with pandemic H1N1, seasonal H1N1 and H3N2 viruses.
Article in Virology journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers, 4 of them syntheses that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
45 citing papers in PubMed, 4 syntheses or guidelines pooled it, 93 citations in OpenAlex.
- Pooled it
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- Comparison of influenza viral load in nasopharyngeal and midturbinate swabs.Influenza and other respiratory viruses · 2024Trial
- Influenza.Nature reviews. Disease primers · 2026Review
- Validation of sample pooling for high-throughput RT-qPCR subtyping of avian influenza A(H5).Microbiology spectrum · 2026Article
- Point-of-need one-pot multiplexed RT-LAMP test for detecting three common respiratory viruses in saliva.Biosensors & bioelectronics · 2025Article
- Saliva and Anterior Nasal Samples for Detecting Respiratory Viruses in Children.APMIS : acta pathologica, microbiologica, et immunologica Scandinavica · 2025Article
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- Key Insights into Respiratory Virus Testing: Sensitivity and Clinical Implications.Microorganisms · 2025Review
- Taiwanese Guidelines for Molecular Point-of-Care Testing for Influenza in Emergency Medicine From the New Diagnostic Technology Team of the Taiwan Society of Emergency Medicine.Journal of acute medicine · 2023Article
- Transmission and pathogenicity of canine H3N2 influenza virus in dog and guinea pig models.Virology journal · 2022Article
- In vitro testing of salt coating of fabrics as a potential antiviral agent in reusable face masks.Scientific reports · 2022Article
- Requirements and Study Designs for U.S. Regulatory Approval of Influenza Home Tests.Journal of clinical microbiology · 2022Review
- Decontamination of High-Efficiency Mask Filters From Respiratory Pathogens Including SARS-CoV-2 by Non-thermal Plasma.Frontiers in bioengineering and biotechnology · 2022Article
- Nucleic Acid-Based Sensing Techniques for Diagnostics and Surveillance of Influenza.Biosensors · 2021Review
- Positive tracheal SARS-CoV-2 RNA test after three negative SARS-CoV-2 RNA tests in a patient with COVID-19.Canadian journal of anaesthesia = Journal canadien d'anesthesie · 2020Article
- Associations Between Relative Viral Load at Diagnosis and Influenza A Symptoms and Recovery.Open forum infectious diseases · 2020Article
- SARS-CoV-2 in wastewater: State of the knowledge and research needs.The Science of the total environment · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundNasopharyngeal aspirate (NPA), nasal swab (NS), and throat swab (TS) are common specimens used for diagnosis of respiratory virus infections based on the detection of viral genomes, viral antigens and viral isolation. However, there is no documented data regarding the type of specimen that yields the best result of viral detection. In this study, quantitative real time RT-PCR specific for M gene was used to determine influenza A viral loads present in NS, NPA and TS samples collected from patients infected with the 2009 pandemic H1N1, seasonal H1N1 and H3N2 viruses. Various copy numbers of RNA transcripts derived from recombinant plasmids containing complete M gene insert of each virus strain were assayed by RT-PCR. A standard curve for viral RNA quantification was constructed by plotting each Ct value against the log quantity of each standard RNA copy number.
resultsCopy numbers of M gene were obtained through the extrapolation of Ct values of the test samples against the corresponding standard curve. Among a total of 29 patients with severe influenza enrolled in this study (12 cases of the 2009 pandemic influenza, 5 cases of seasonal H1N1 and 12 cases of seasonal H3N2 virus), NPA was found to contain significantly highest amount of viral loads and followed in order by NS and TS specimen. Viral loads among patients infected with those viruses were comparable regarding type of specimen analyzed.
conclusionBased on M gene copy numbers, we conclude that NPA is the best specimen for detection of influenza A viruses, and followed in order by NS and TS.
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Registered trials
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