ArticleBMC infectious diseases2010
Comparison of commercial DNA preparation kits for the detection of Brucellae in tissue using quantitative real-time PCR.
Article in BMC infectious diseases, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 43 citations in OpenAlex.
- A critical review of diagnostic methods for camel brucellosis.Veterinary and animal science · 2026Review
- Two novel real-time PCR assays forMicrobiology spectrum · 2026Article
- Fatal acute undifferentiated febrile illness among clinically suspected leptospirosis cases in Colombia, 2016-2019.PLoS neglected tropical diseases · 2023Article
- A rapid and simple bead-bashing-based method for genomic DNA extraction from mammalian tissue.BioTechniques · 2020Article
- Laboratory Diagnosis of Human Brucellosis.Clinical microbiology reviews · 2019Review
- Answer to February 2018 Photo Quiz.Journal of clinical microbiology · 2018Article
- Detection of Francisella tularensis in voles in Finland.Vector borne and zoonotic diseases (Larchmont, N.Y.) · 2014Article
- Detection and identification of Legionella species in hospital water supplies through Polymerase Chain Reaction (16S rRNA).Journal of environmental health science & engineering · 2014Article
- Broad-range real-time PCR assay for detection of bacterial DNA in ocular samples from infectious endophthalmitis.Japanese journal of ophthalmology · 2012Article
- An optimized real-time PCR to avoid species-/tissue-associated inhibition for H5N1 detection in ferret and monkey tissues.TheScientificWorldJournal · 2012Article
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Authors and funding
8 authors at 7 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe detection of Brucellae in tissue specimens using PCR assays is difficult because the amount of bacteria is usually low. Therefore, optimised DNA extraction methods are critical. The aim of this study was to assess the performance of commercial kits for the extraction of Brucella DNA.
methodsFive kits were evaluated using clinical specimens: QIAamp DNA Mini Kit (QIAGEN), peqGold Tissue DNA Mini Kit (PeqLab), UltraClean Tissue and Cells DNA Isolation Kit (MoBio), DNA Isolation Kit for Cells and Tissues (Roche), and NucleoSpin Tissue (Macherey-Nagel). DNA yield was determined using a quantitative real-time PCR assay targeting IS711 that included an internal amplification control.
resultsKits of QIAGEN and Roche provided the highest amount of DNA, Macherey-Nagel and Peqlab products were intermediate whereas MoBio yielded the lowest amount of DNA. Differences were significant (p < 0.05) and of diagnostic relevance. Sample volume, elution volume, and processing time were also compared.
conclusionsWe observed differences in DNA yield as high as two orders of magnitude for some samples between the best and the worst DNA extraction kits and inhibition was observed occasionally. This indicates that DNA purification may be more relevant than expected when the amount of DNA in tissue is very low.
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