Evidence map›Paper›PMID 20401502›Full record

ArticleExtremophiles : life under extreme conditions2010

Isolation and characterisation of bacteria from the haloalkaline Lake Elmenteita, Kenya.

Romano Mwirichia, A W Muigai, B Tindall, H I Boga, E Stackebrandt

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Article in Extremophiles : life under extreme conditions, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

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17citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

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3 · Its place in the literature

Who cites it

17 citing papers in PubMed.

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  3. Bioprospecting potentials of moderately halophilic bacteria and the isolation of squalene producers from Kuwait sabkha.International microbiology : the official journal of the Spanish Society for Microbiology · 2021
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  4. International journal of systematic and evolutionary microbiology · 2021
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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Romano MwirichiaInstitute for Biotechnology Research, Jomo Kenyatta University of Agriculture and Technology, P.O. Box 62000, Nairobi, Kenya. romano.mwirichia@yahoo.com
A W Muigai
B Tindall
H I Boga
E Stackebrandt

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Culture-independent studies show that soda lake environments harbour diverse groups of bacteria and archaea. In this study different enrichment and isolation media were used in an attempt to isolate novel groups of bacteria from Lake Elmenteita. Different media were prepared using filter-sterilised water from the lake. The isolates recovered were purified on tryptic soy agar supplemented with 1% sodium carbonate and 4% sodium chloride. Phylogenetic analysis of 181 partial 16S rRNA gene sequences with excellent quality showed that the majority of the isolates were affiliated to the class Gammaproteobacteria and to the genus Bacillus. Isolates from the genus Halomonas and Bacillus constituted 37 and 31% of the total sequenced isolates, respectively. Other groups recovered were related to Marinospirillum, Idiomarina, Vibrio, Enterococcus, Alkalimonas, Alkalibacterium, Amphibacillus, Marinilactibacillus and the actinobacteria Nocardiopsis and Streptomyces. Fifty-one different genera were represented with 31 and 15 cultures scoring with their nearest neighbour similarities below 98 and 97%, respectively. Some novel taxa were identified which had not been isolated previously from the soda environment. The results show that the use of different media with varying compositions can help retrieve novel bacterial diversity from the soda lake environment.

Indexed as

Water MicrobiologyBacteriaBase SequenceDNA PrimersFresh WaterKenyaPhylogenyDNA Primers

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.