ArticleThe Plant cell2010
RNA-dependent RNA polymerase 1 from Nicotiana tabacum suppresses RNA silencing and enhances viral infection in Nicotiana benthamiana.
Article in The Plant cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed, 109 citations in OpenAlex.
- Handmade cloned transgenic piglets expressing the nematode fat-1 gene.Cellular reprogramming · 2012Trial
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- Transient Expression of Phytoplasma Proteins in Planta Using an Agro-Infiltration Method.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Viral RNA pUGylation promotes antiviral immunity inJournal of virology · 2025Article
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- Crop antiviral defense: Past and future perspective.Science China. Life sciences · 2024Review
- Conservation of molecular responses upon viral infection in the non-vascular plant Marchantia polymorpha.Nature communications · 2024Article
- Deficiency of multiple RNA silencing-associated genes may contribute to the increased susceptibility of Nicotiana benthamiana to viruses.Plant cell reports · 2024Article
- Near-infrared light and PIF4 promote plant antiviral defense by enhancing RNA interference.Plant communications · 2024Article
- Article
- Thermosensitive SUMOylation of TaHsfA1 defines a dynamic ON/OFF molecular switch for the heat stress response in wheat.The Plant cell · 2023Article
- A persistent major mutation in canonical jasmonate signaling is embedded in an herbivory-elicited gene network.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Article
- Transgenerational Tolerance to Salt and Osmotic Stresses Induced by Plant Virus Infection.International journal of molecular sciences · 2022Article
- Analysis of the RNA-Dependent RNA Polymerase 1 (RDR1) Gene Family in Melon.Plants (Basel, Switzerland) · 2022Article
- The Carboxyl Terminal Regions of P0 Protein Are Required for Systemic Infections of Poleroviruses.International journal of molecular sciences · 2022Article
- Cucumber RDR1s and cucumber mosaic virus suppressor protein 2b association directs host defence in cucumber plants.Molecular plant pathology · 2021Article
- Natural Resources Resistance to Tomato Spotted Wilt Virus (TSWV) in Tomato (International journal of molecular sciences · 2021Review
- RNA-dependent RNA polymerase 1 delays the accumulation of viroids in infected plants.Molecular plant pathology · 2021Article
- Resistance induction based on the understanding of molecular interactions between plant viruses and host plants.Virology journal · 2021Review
3 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Endogenous eukaryotic RNA-dependent RNA polymerases (RDRs) produce double-stranded RNA intermediates in diverse processes of small RNA synthesis in RNA silencing pathways. RDR6 is required in plants for posttranscriptional gene silencing induced by sense transgenes (S-PTGS) and has an important role in amplification of antiviral silencing. Whereas RDR1 is also involved in antiviral defense in plants, this does not necessarily proceed through triggering silencing. In this study, we show that Nicotiana benthamiana transformed with RDR1 from Nicotiana tabacum (Nt-RDR1 plants) exhibits hypersusceptibility to Plum pox potyvirus and other viruses, resembling RDR6-silenced (RDR6i) N. benthamiana. Analysis of transient induction of RNA silencing in N. benthamiana Nt-RDR1 and RDR6i plants revealed that Nt-RDR1 possesses silencing suppression activity. We found that Nt-RDR1 does not interfere with RDR6-dependent siRNA accumulation but turns out to suppress RDR6-dependent S-PTGS. Our results, together with previously published data, suggest that RDR1 might have a dual role, contributing, on one hand, to salicylic acid-mediated antiviral defense, and suppressing, on the other hand, the RDR6-mediated antiviral RNA silencing. We propose a scenario in which the natural loss-of-function variant of RDR1 in N. benthamiana may be the outcome of selective pressure to maintain a high RDR6-dependent antiviral defense, which would be required to face the hypersensitivity of this plant to a large number of viruses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.