ArticleApplied and environmental microbiology2010
Expression and function of four carbonic anhydrase homologs in the deep-sea chemolithoautotroph Thiomicrospira crunogena.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- Unusual regulation of the COApplied and environmental microbiology · 2025Article
- Overexpression of native carbonic anhydrases increases carbon conversion efficiency in the methanotrophic biocatalystmSphere · 2024Article
- Widespread dissolved inorganic carbon-modifying toolkits in genomes of autotrophicApplied and environmental microbiology · 2024Review
- Comparative genomic insights into habitat adaptation of coral-associatedFrontiers in microbiology · 2023Article
- The MpsAB Bicarbonate Transporter Is Superior to Carbonic Anhydrase in Biofilm-Forming Bacteria with Limited COMicrobiology spectrum · 2021Article
- Involvement of β-Carbonic Anhydrase Genes in Bacterial Genomic Islands and Their Horizontal Transfer to Protists.Applied and environmental microbiology · 2018Article
- Regulatory components of carbon concentrating mechanisms in aquatic unicellular photosynthetic organisms.Plant cell reports · 2017Review
- Proteomic and Mutant Analysis of the COJournal of bacteriology · 2017Article
- Bacterial Community Dynamics and Biocement Formation during Stimulation and Augmentation: Implications for Soil Consolidation.Frontiers in microbiology · 2017Article
- Activity and anion inhibition studies of the α-carbonic anhydrase from Thiomicrospira crunogena XCL-2 Gammaproteobacterium.Bioorganic & medicinal chemistry letters · 2015Article
- Structural and biophysical characterization of the α-carbonic anhydrase from the gammaproteobacterium Thiomicrospira crunogena XCL-2: insights into engineering thermostable enzymes for CO2 sequestration.Acta crystallographica. Section D, Biological crystallography · 2015Article
- A novel hydrogen oxidizer amidst the sulfur-oxidizing Thiomicrospira lineage.The ISME journal · 2015Article
- Insights into bacterial CO2 metabolism revealed by the characterization of four carbonic anhydrases in Ralstonia eutropha H16.AMB Express · 2014Article
- Three functional β-carbonic anhydrases in Pseudomonas aeruginosa PAO1: role in survival in ambient air.Microbiology (Reading, England) · 2013Article
- Preliminary X-ray crystallographic analysis of α-carbonic anhydrase from Thiomicrospira crunogena XCL-2.Acta crystallographica. Section F, Structural biology and crystallization communications · 2012Article
- Transcriptional response of the sulfur chemolithoautotroph Thiomicrospira crunogena to dissolved inorganic carbon limitation.Journal of bacteriology · 2012Article
- Carboxysomes: cyanobacterial RubisCO comes in small packages.Photosynthesis research · 2011Review
- The biological deep sea hydrothermal vent as a model to study carbon dioxide capturing enzymes.Marine drugs · 2011Review
- Metagenomic comparison of two Thiomicrospira lineages inhabiting contrasting deep-sea hydrothermal environments.PloS one · 2010Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The hydrothermal vent chemolithoautotroph Thiomicrospira crunogena grows rapidly in the presence of low concentrations of dissolved inorganic carbon (DIC) (= CO(2) + HCO(3)(-) + CO(3)(-2)). Its genome encodes alpha-carbonic anhydrase (alpha-CA), beta-CA, carboxysomal beta-like CA (CsoSCA), and a protein distantly related to gamma-CA. The purposes of this work were to characterize the gene products, determine whether they were differentially expressed, and identify those that are necessary for DIC uptake and fixation. When expressed in Escherichia coli, CA activity was detectable for alpha-CA, beta-CA, and CsoSCA but not for the gamma-CA-like protein. alpha-CA and CsoSCA but not beta-CA were inhibited by sulfonamide inhibitors. CsoSCA was also inhibited by dithiothreitol. When grown under DIC limitation in chemostats, T. crunogena transcribed csoSCA more frequently than when ammonia limited, while genes encoding alpha-CA and beta-CA were not differentially transcribed under these conditions. Cell extracts from T. crunogena grown under both DIC- and ammonia-limited conditions had CA activity that was strongly inhibited by sulfonamides, though extracts from nitrogen-limited cells had some CA activity that was resistant, perhaps due to a higher level of beta-CA activity. Based on predictions from the SignalP software program, subcellular location when expressed in E. coli, and carbonic anhydrase assays conducted on intact T. crunogena cells, alpha-CA is located in the periplasm. However, inhibition of alpha-CA by acetazolamide had only a minor impact on rates of DIC uptake or fixation. Conversely, inhibition of CsoSCA with ethoxyzolamide inhibited carbon fixation but not DIC uptake, consistent with this enzyme functioning to facilitate DIC interconversion and fixation within carboxysomes.
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