ArticleApplied and environmental microbiology2010
Purification and characterization of OmcZ, an outer-surface, octaheme c-type cytochrome essential for optimal current production by Geobacter sulfurreducens.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
75 citing papers in PubMed, 258 citations in OpenAlex.
- Comparative transcriptomics and scarless genome editing uncover a periplasmicApplied and environmental microbiology · 2026Article
- Expression and Engineering of Conductive Cytochrome Nanowires.bioRxiv : the preprint server for biology · 2026Article
- Synthesis and biophysical characterization of bioengineered cytochrome nanowires.European biophysics journal : EBJ · 2026Article
- Phylogenomic and metabolic insights into iron reduction metabolism in the genusMicrobial genomics · 2026Article
- Evolution for enhanced extracellular electron transfer inFrontiers in microbiology · 2026Article
- Genetically Reprogramming Crops and Rhizobacteria for Nutritional Iron Biofortification.ACS synthetic biology · 2025Review
- Redox conduction facilitates direct interspecies electron transport in anaerobic methanotrophic consortia.Science advances · 2025Article
- Influence of support materials on the electroactive behavior, structure and gene expression of wild type and GSU1771-deficient mutant of Geobacter sulfurreducens biofilms.Environmental science and pollution research international · 2025Article
- The Role of Anode Potential in Electromicrobiology.Microorganisms · 2025Review
- Electron transfer in multicentre redox proteins: from fundamentals to extracellular electron transfer.Bioscience reports · 2025Review
- Subcellular thiol functional group distribution inFrontiers in microbiology · 2025Article
- Prediction and validation of nanowire proteins inComputational and structural biotechnology journal · 2025Article
- Periplasmic electron transfer network in Geobacter sulfurreducens revealed by biomolecular interaction studies.Protein science : a publication of the Protein Society · 2024Article
- Potential coupling of microbial methane, nitrogen, and sulphur cycling in the Okinawa Trough cold seep sediments.Microbiology spectrum · 2024Article
- MmcA is an electron conduit that facilitates both intracellular and extracellular electron transport in Methanosarcina acetivorans.Nature communications · 2024Article
- Widespread extracellular electron transfer pathways for charging microbial cytochrome OmcS nanowires via periplasmic cytochromes PpcABCDE.Nature communications · 2024Article
- Multi-heme cytochrome-mediated extracellular electron transfer by the anaerobic methanotroph 'Candidatus Methanoperedens nitroreducens'.Nature communications · 2023Article
- MmcA is an electron conduit that facilitates both intracellular and extracellular electron transport inbioRxiv : the preprint server for biology · 2023Article
- Enhancing electrical outputs of the fuel cells with Geobacter sulferreducens by overexpressing nanowire proteins.Microbial biotechnology · 2023Article
- Structure of Geobacter cytochrome OmcZ identifies mechanism of nanowire assembly and conductivity.Nature microbiology · 2023Article
15 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previous studies have demonstrated that Geobacter sulfurreducens requires the c-type cytochrome OmcZ, which is present in large (OmcZ(L); 50-kDa) and small (OmcZ(S); 30-kDa) forms, for optimal current production in microbial fuel cells. This protein was further characterized to aid in understanding its role in current production. Subcellular-localization studies suggested that OmcZ(S) was the predominant extracellular form of OmcZ. N- and C-terminal amino acid sequence analysis of purified OmcZ(S) and molecular weight measurements indicated that OmcZ(S) is a cleaved product of OmcZ(L) retaining all 8 hemes, including 1 heme with the unusual c-type heme-binding motif CX(14)CH. The purified OmcZ(S) was remarkably thermally stable (thermal-denaturing temperature, 94.2 degrees C). Redox titration analysis revealed that the midpoint reduction potential of OmcZ(S) is approximately -220 mV (versus the standard hydrogen electrode [SHE]) with nonequivalent heme groups that cover a large reduction potential range (-420 to -60 mV). OmcZ(S) transferred electrons in vitro to a diversity of potential extracellular electron acceptors, such as Fe(III) citrate, U(VI), Cr(VI), Au(III), Mn(IV) oxide, and the humic substance analogue anthraquinone-2,6-disulfonate, but not Fe(III) oxide. The biochemical properties and extracellular localization of OmcZ suggest that it is well suited for promoting electron transfer in current-producing biofilms of G. sulfurreducens.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.