ArticleApplied and environmental microbiology2010
Development of a chip assay and quantitative PCR for detecting microcystin synthetase E gene expression.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 82 citations in OpenAlex.
- Bacterial and microeukaryotic assemblage transitions during a harmful algal bloom in the Nakdong River.FEMS microbiology ecology · 2026Article
- Investigation of cyanobacteria-hosted antibiotic resistance genes in cyanoHAB-impacted drinking water sources.Environmental science and pollution research international · 2026Article
- Dominant Dolichospermum and microcystin production in Detroit Lake (Oregon, USA).Harmful algae · 2025Article
- Article
- Cyanotoxins, biosynthetic gene clusters, and factors modulating cyanotoxin biosynthesis.World journal of microbiology & biotechnology · 2023Review
- Cyanobacterial Algal Bloom Monitoring: Molecular Methods and Technologies for Freshwater Ecosystems.Microorganisms · 2023Review
- Microbial communities in aerosol generated from cyanobacterial bloom-affected freshwater bodies: an exploratory study in Nakdong River, South Korea.Frontiers in microbiology · 2023Article
- Article
- Characterization and Diversity of Microcystins Produced by Cyanobacteria from the Curonian Lagoon (SE Baltic Sea).Toxins · 2021Article
- Depth-Dependent Spatiotemporal Dynamics of Overwintering PelagicMicroorganisms · 2021Article
- Prognostic biomarkers and therapeutic targets in oral squamous cell carcinoma: a study based on cross-database analysis.Hereditas · 2021Article
- Predicting microcystin concentration action-level exceedances resulting from cyanobacterial blooms in selected lake sites in Ohio.Environmental monitoring and assessment · 2020Article
- Using Microcystin Gene Copies to Determine Potentially-Toxic Blooms, Example from a Shallow Eutrophic Lake Peipsi.Toxins · 2020Article
- Use of qPCR and RT-qPCR for monitoring variations of microcystin producers and as an early warning system to predict toxin production in an Ohio inland lake.Water research · 2020Article
- Democratizing water monitoring: Implementation of a community-based qPCR monitoring program for recreational water hazards.PloS one · 2020Article
- Phytoplankton community and algal toxicity at a recurring bloom in Sullivan Bay, Kabetogama Lake, Minnesota, USA.Scientific reports · 2019Article
- Article
- Detection of microcystin-producing cyanobacteria in water samples using loop-mediated isothermal amplification targeting3 Biotech · 2018Article
- Influence of temperature, mixing, and addition of microcystin-LR on microcystin gene expression in Microcystis aeruginosa.MicrobiologyOpen · 2017Article
- Temporal Dynamics of the Microbial Community Composition with a Focus on Toxic Cyanobacteria and Toxin Presence during Harmful Algal Blooms in Two South German Lakes.Frontiers in microbiology · 2017Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The chip and quantitative real-time PCR (qPCR) assays were optimized to study the expression of microcystin biosynthesis genes (mcy) with RNA samples extracted from cyanobacterial strains and environmental water samples. Both microcystin-producing Anabaena and Microcystis were identified in Lake Tuusulanjärvi samples. Microcystis transcribed the mcyE genes throughout the summer of 2006, while expression by Anabaena became evident later in August and September. Active mcyE gene expression was also detectable when microcystin concentrations were very low. Detection of Anabaena mcyE transcripts by qPCR, as well as certain cyanobacterial 16S rRNAs with the chip assay, showed slightly reduced sensitivity compared with the DNA analyses. In contrast, even groups undetectable or present in low quantities as determined by microscopy could be identified with the chip assay from DNA samples. The methods introduced add to the previously scarce repertoire of applications for mcy expression profiling in environmental samples and enable in situ studies of regulation of microcystin synthesis in response to environmental factors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.