ArticleApplied and environmental microbiology2010
Contribution of a xylan-binding module to the degradation of a complex cellulosic substrate by designer cellulosomes.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 62 citations in OpenAlex.
- The Microbial Community Succession Drives Stage-Specific Carbon Metabolic Shifts DuringMicroorganisms · 2025Article
- Unique Fn3-like biosensor in σProtein science : a publication of the Protein Society · 2024Article
- Conversion of the free Cellvibrio japonicus xyloglucan degradation system to the cellulosomal mode.Applied microbiology and biotechnology · 2022Article
- Changing surface grafting density has an effect on the activity of immobilized xylanase towards natural polysaccharides.Scientific reports · 2019Article
- Optimizing the composition of a synthetic cellulosome complex for the hydrolysis of softwood pulp: identification of the enzymatic core functions and biochemical complex characterization.Biotechnology for biofuels · 2018Article
- Toward combined delignification and saccharification of wheat straw by a laccase-containing designer cellulosome.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Adaptor Scaffoldins: An Original Strategy for Extended Designer Cellulosomes, Inspired from Nature.mBio · 2016Article
- Enhancement of cellulosome-mediated deconstruction of cellulose by improving enzyme thermostability.Biotechnology for biofuels · 2016Article
- Cellulosomal expansin: functionality and incorporation into the complex.Biotechnology for biofuels · 2016Article
- Crucial roles of single residues in binding affinity, specificity, and promiscuity in the cellulosomal cohesin-dockerin interface.The Journal of biological chemistry · 2015Article
- Significance of relative position of cellulases in designer cellulosomes for optimized cellulolysis.PloS one · 2015Article
- Contribution of a family 1 carbohydrate-binding module in thermostable glycoside hydrolase 10 xylanase from Talaromyces cellulolyticus toward synergistic enzymatic hydrolysis of lignocellulose.Biotechnology for biofuels · 2015Article
- The contribution of cellulosomal scaffoldins to cellulose hydrolysis by Clostridium thermocellum analyzed by using thermotargetrons.Biotechnology for biofuels · 2014Article
- A combined cell-consortium approach for lignocellulose degradation by specialized Lactobacillus plantarum cells.Biotechnology for biofuels · 2014Article
- A synthetic biology approach for evaluating the functional contribution of designer cellulosome components to deconstruction of cellulosic substrates.Biotechnology for biofuels · 2013Article
- A novel neutral xylanase with high SDS resistance from Volvariella volvacea: characterization and its synergistic hydrolysis of wheat bran with acetyl xylan esterase.Journal of industrial microbiology & biotechnology · 2013Article
- Establishment of a simple Lactobacillus plantarum cell consortium for cellulase-xylanase synergistic interactions.Applied and environmental microbiology · 2013Article
- Development and biotechnological application of a novel endoxylanase family GH10 identified from sugarcane soil metagenome.PloS one · 2013Article
- Deconstruction of lignocellulose into soluble sugars by native and designer cellulosomes.mBio · 2012Article
- Paradigmatic status of an endo- and exoglucanase and its effect on crystalline cellulose degradation.Biotechnology for biofuels · 2012Article
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Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Conversion of components of the Thermobifida fusca free-enzyme system to the cellulosomal mode using the designer cellulosome approach can be employed to discover the properties and inherent advantages of the cellulosome system. In this article, we describe the conversion of the T. fusca xylanases Xyn11A and Xyn10B and their synergistic interaction in the free state or within designer cellulosome complexes in order to enhance specific degradation of hatched wheat straw as a model for a complex cellulosic substrate. Endoglucanase Cel5A from the same bacterium and its recombinant dockerin-containing chimera were also studied for their combined effect, together with the xylanases, on straw degradation. Synergism was demonstrated when Xyn11A was combined with Xyn10B and/or Cel5A, and approximately 1.5-fold activity enhancements were achieved by the designer cellulosome complexes compared to the free wild-type enzymes. These improvements in activity were due to both substrate-targeting and proximity effects among the enzymes contained in the designer cellulosome complexes. The intrinsic cellulose/xylan-binding module (XBM) of Xyn11A appeared to be essential for efficient substrate degradation. Indeed, only designer cellulosomes in which the XBM was maintained as a component of Xyn11A achieved marked enhancement in activity compared to the combination of wild-type enzymes. Moreover, integration of the XBM in designer cellulosomes via a dockerin module (separate from the Xyn11A catalytic module) failed to enhance activity, suggesting a role in orienting the parent xylanase toward its preferred polysaccharide component of the complex wheat straw substrate. The results provide novel mechanistic insight into the synergistic activity of designer cellulosome components on natural plant cell wall substrates.
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