ArticleJournal of structural biology2010
Ion-abrasion scanning electron microscopy reveals distorted liver mitochondrial morphology in murine methylmalonic acidemia.
Article in Journal of structural biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
- An interactive deep learning-based approach reveals mitochondrial cristae topologies.PLoS biology · 2023Article
- Review
- Instance segmentation of mitochondria in electron microscopy images with a generalist deep learning model trained on a diverse dataset.Cell systems · 2023Article
- Enzymatically dissociated muscle fibers display rapid dedifferentiation and impaired mitochondrial calcium control.iScience · 2022Article
- Mitochondrial disease, mitophagy, and cellular distress in methylmalonic acidemia.Cellular and molecular life sciences : CMLS · 2021Review
- Gene Therapy for Methylmalonic Acidemia: Past, Present, and Future.Human gene therapy · 2019Review
- Subcellular connectomic analyses of energy networks in striated muscle.Nature communications · 2018Article
- Inter-relations between 3-hydroxypropionate and propionate metabolism in rat liver: relevance to disorders of propionyl-CoA metabolism.American journal of physiology. Endocrinology and metabolism · 2017Article
- Glutathione as a Redox Biomarker in Mitochondrial Disease-Implications for Therapy.Journal of clinical medicine · 2017Review
- Methylmalonic and propionic acidemias: clinical management update.Current opinion in pediatrics · 2016Review
- Focused ion beams in biology.Nature methods · 2015Review
- In vivo microscopy reveals extensive embedding of capillaries within the sarcolemma of skeletal muscle fibers.Microcirculation (New York, N.Y. : 1994) · 2014Article
- Bioanalysis of eukaryotic organelles.Chemical reviews · 2013Review
- Analysis of lipid droplets in cardiac muscle.Methods in cell biology · 2013Article
- Advances in high-resolution imaging--techniques for three-dimensional imaging of cellular structures.Journal of cell science · 2012Review
- Electron tomography of cells.Quarterly reviews of biophysics · 2012Review
- Correlative 3D imaging of whole mammalian cells with light and electron microscopy.Journal of structural biology · 2011Article
- Mitochondrial energy metabolism in neurodegeneration associated with methylmalonic acidemia.Journal of bioenergetics and biomembranes · 2011Review
- The origins and evolution of freeze-etch electron microscopy.Journal of electron microscopy · 2011Review
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 1 country.
Funding
Abstract
Methylmalonic acidemia is a lethal inborn error of metabolism that causes mitochondrial impairment, multi-organ dysfunction and a shortened lifespan. Previous transmission electron microscope studies of thin sections from normal (Mut(+/+)) and diseased (Mut(-/-)) tissue found that the mitochondria appear to occupy a progressively larger volume of mutant cells with age, becoming megamitochondria. To assess changes in shape and volume of mitochondria resulting from the mutation, we carried out ion-abrasion scanning electron microscopy (IA-SEM), a method for 3D imaging that involves the iterative use of a focused gallium ion beam to abrade the surface of the specimen, and a scanning electron beam to image the newly exposed surface. Using IA-SEM, we show that mitochondria are more convoluted and have a broader distribution of sizes in the mutant tissue. Compared to normal cells, mitochondria from mutant cells have a larger surface-area-to-volume ratio, which can be attributed to their convoluted shape and not to their elongation or reduced volume. The 3D imaging approach and image analysis described here could therefore be useful as a diagnostic tool for the evaluation of disease progression in aberrant cells at resolutions higher than that currently achieved using confocal light microscopy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.