ArticleCurrent biology : CB2010
Motor number controls cargo switching at actin-microtubule intersections in vitro.
Article in Current biology : CB, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 92 citations in OpenAlex.
- Temporal Patterns of Angular Displacement of Endosomes: Insights into Motor Protein Exchange Dynamics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Presynaptic perspective: Axonal transport defects in neurodevelopmental disorders.The Journal of cell biology · 2024Review
- Article
- Vesicle-Associated Actin Assembly by Formins Promotes TGFβ-Induced ANGPTL4 Trafficking, Secretion and Cell Invasion.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Cargo properties play a critical role in myosin Va-driven cargo transport along actin filaments.Biochemistry and biophysics reports · 2022Article
- New twists in actin-microtubule interactions.Molecular biology of the cell · 2021Review
- Modelling cytoskeletal transport by clusters of non-processive molecular motors with limited binding sites.Royal Society open science · 2020Article
- The ability of the kinesin-2 heterodimer KIF3AC to navigate microtubule networks is provided by the KIF3A motor domain.The Journal of biological chemistry · 2019Article
- Cargos Rotate at Microtubule Intersections during Intracellular Trafficking.Biophysical journal · 2018Article
- Engaging myosin VI tunes motility, morphology and identity in endocytosis.Traffic (Copenhagen, Denmark) · 2018Article
- Capture of Dense Core Vesicles at Synapses by JNK-Dependent Phosphorylation of Synaptotagmin-4.Cell reports · 2017Article
- Small teams of myosin Vc motors coordinate their stepping for efficient cargo transport on actin bundles.The Journal of biological chemistry · 2017Article
- Transport of fungal RAB11 secretory vesicles involves myosin-5, dynein/dynactin/p25, and kinesin-1 and is independent of kinesin-3.Molecular biology of the cell · 2017Article
- Cargo Transport by Two Coupled Myosin Va Motors on Actin Filaments and Bundles.Biophysical journal · 2016Article
- A Genome-wide RNAi Screen for Microtubule Bundle Formation and Lysosome Motility Regulation in Drosophila S2 Cells.Cell reports · 2016Article
- Collective dynamics of processive cytoskeletal motors.Soft matter · 2016Review
- Bidirectional actin transport is influenced by microtubule and actin stability.Cellular and molecular life sciences : CMLS · 2015Article
- Biogenesis of the crystalloid organelle in Plasmodium involves microtubule-dependent vesicle transport and assembly.International journal for parasitology · 2015Article
- Control of the initiation and termination of kinesin-1-driven transport by myosin-Ic and nonmuscle tropomyosin.Current biology : CB · 2015Article
- A method for multiprotein assembly in cells reveals independent action of kinesins in complex.The Journal of cell biology · 2014Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
backgroundCellular activities such as endocytosis and secretion require that cargos actively switch between the microtubule (MT) and actin filament (AF) networks. Cellular studies suggest that switching may involve a tug of war or coordinate regulation of MT- and AF-based motor function.
resultsTo test the hypothesis that motor number can be used to direct the outcome of a tug-of-war process, we reconstituted cargo switching at MT-AF intersections in a minimal system with purified myosin V and dynein-dynactin motors bound to beads. Beads containing both motors often paused at the intersections and rotated about an axis perpendicular to both filaments, suggesting that competing motors apply a torque on their cargo. Force measurements showed that motor forces scale with the number of engaged myosin V and dynein-dynactin motors. Whether beads remained on a MT or AF or switched to the alternate track was determined by which set of motors collectively produced greater force. Passing and switching probabilities were similar whether the bead approached an intersection on either a MT or an AF. Beads with a force ratio near unity had approximately equal probabilities of exiting on the MT, exiting on the AF, or remaining stalled at the intersection. A simple statistical model quantitatively describes the relationship between switching probability and motor number.
conclusionsCargo switching can be tuned via combinations of 1-4 myosin V and 1-4 dynein-dynactin engaged motors through a simple force-mediated mechanism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.