ArticleExperimental eye research2010
Genetic deletion of COX-2 diminishes VEGF production in mouse retinal Müller cells.
Article in Experimental eye research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 50 citations in OpenAlex.
- Breakthroughs in diabetic retinopathy diagnosis and treatment using preclinical research models: current progress and future directions.Annals of medicine · 2025Review
- Eicosapentaenoic Acid (EPA) and Docosahexaenoic Acid (DHA): A Targeted Antioxidant Strategy to Counter Oxidative Stress in Retinopathy.Antioxidants (Basel, Switzerland) · 2024Review
- Transition metals in angiogenesis - A narrative review.Materials today. Bio · 2023Review
- Elucidating glial responses to products of diabetes-associated systemic dyshomeostasis.Progress in retinal and eye research · 2023Review
- Cyclooxygenase inhibition attenuates brain angiogenesis and independently decreases mouse survival under hypoxia.Journal of neurochemistry · 2021Article
- Topical administration of the kappa opioid receptor agonist nalfurafine suppresses corneal neovascularization and inflammation.Scientific reports · 2021Article
- Visualizing HIF-1α mRNA in a Subpopulation of Bone Marrow-Derived Cells to Predict Retinal Neovascularization.ACS chemical biology · 2020Article
- Lipid Signaling in Ocular Neovascularization.International journal of molecular sciences · 2020Review
- Combination Antioxidant/NSAID Therapies and Oral/Topical Ocular Delivery Modes for Prevention of Oxygen-Induced Retinopathy in a Rat Model.Nutrients · 2020Article
- Mapping the structure and biological functions within mesenchymal bodies using microfluidics.Science advances · 2020Article
- Role of aldose reductase in diabetes-induced retinal microglia activation.Chemico-biological interactions · 2019Article
- Extract of the Blood Circulation-Promoting Recipe-84 Can Protect Rat Retinas by Inhibiting the β-Catenin Signaling Pathway.International journal of molecular sciences · 2018Article
- Viral Retinopathy in Experimental Models of Zika Infection.Investigative ophthalmology & visual science · 2017Article
- Gender differences in hypoxic acclimatization in cyclooxygenase-2-deficient mice.Physiological reports · 2017Article
- Fluorocoxib A enables targeted detection of cyclooxygenase-2 in laser-induced choroidal neovascularization.Journal of biomedical optics · 2016Article
- Molecular Markers of Diabetic Retinopathy: Potential Screening Tool of the Future?Frontiers in physiology · 2016Review
- HSPA12B: a novel facilitator of lung tumor growth.Oncotarget · 2015Article
- Applications of azo-based probes for imaging retinal hypoxia.ACS medicinal chemistry letters · 2015Article
- Celecoxib attenuates retinal angiogenesis in a mouse model of oxygen-induced retinopathy.International journal of clinical and experimental pathology · 2015Article
- Mitigation of oxygen-induced retinopathy in α2β1 integrin-deficient mice.Investigative ophthalmology & visual science · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Non-steroidal anti-inflammatory drugs (NSAIDs), which inhibit COX activity, reduce the production of retinal VEGF and neovascularization in relevant models of ocular disease. We hypothesized that COX-2 mediates VEGF production in retinal Müller cells, one of its primary sources in retinal neovascular disease. The purpose of this study was to determine the role of COX-2 and its products in VEGF expression and secretion. These studies have more clearly defined the role of COX-2 and COX-2-derived prostanoids in retinal angiogenesis. Müller cells derived from wild-type and COX-2 null mice were exposed to hypoxia for 0-24 h. COX-2 protein and activity were assessed by western blot analysis and GC-MS, respectively. VEGF production was assessed by ELISA. Wild-type mouse Müller cells were treated with vehicle (0.1% DMSO), 10 microM PGE(2), or PGE(2) + 5 microM H-89 (a PKA inhibitor), for 12 h. VEGF production was assessed by ELISA. Hypoxia significantly increased COX-2 protein (p < 0.05) and activity (p < 0.05), and VEGF production (p < 0.0003). COX-2 null Müller cells produced significantly less VEGF in response to hypoxia (p < 0.05). Of the prostanoids, PGE(2) was significantly increased by hypoxia (p < 0.02). Exogenous PGE(2) significantly increased VEGF production by Müller cells (p < 0.0039), and this effect was inhibited by H-89 (p < 0.055). These data demonstrate that hypoxia induces COX-2, prostanoid production, and VEGF synthesis in Müller cells, and that VEGF production is at least partially COX-2-dependent. Our study suggests that PGE(2), signaling through the EP(2) and/or EP(4) receptor and PKA, mediates the VEGF response of Müller cells.
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Registered trials
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