ArticleBMC microbiology2010
High taxonomic level fingerprint of the human intestinal microbiota by ligase detection reaction--universal array approach.
Article in BMC microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Alterations in fecal microbiota composition by probiotic supplementation in healthy adults: a systematic review of randomized controlled trials.Genome medicine · 2016Pooled it
- A probiotics-containing biscuit modulates the intestinal microbiota in the elderly.The journal of nutrition, health & aging · 2013Trial
- Effects of Different Types of Human Disturbance on Total and Nitrogen-Transforming Bacteria in Haihe River.Life (Basel, Switzerland) · 2022Article
- Characteristics of planktonic and sediment bacterial communities in a heavily polluted urban river.PeerJ · 2021Article
- Considerations for the design and conduct of human gut microbiota intervention studies relating to foods.European journal of nutrition · 2020Review
- Design and validation of a DNA-microarray for phylogenetic analysis of bacterial communities in different oral samples and dental implants.Scientific reports · 2017Article
- Food borne bacterial models for detection of benzo[a]pyrene-DNA adducts formation using RAPD-PCR.Microbial biotechnology · 2016Article
- Dietary Geraniol by Oral or Enema Administration Strongly Reduces Dysbiosis and Systemic Inflammation in Dextran Sulfate Sodium-Treated Mice.Frontiers in pharmacology · 2016Article
- Development of a microarray-based tool to characterize vaginal bacterial fluctuations and application to a novel antibiotic treatment for bacterial vaginosis.Antimicrobial agents and chemotherapy · 2015Article
- Longitudinal analysis of inflammation and microbiota dynamics in a model of mild chronic dextran sulfate sodium-induced colitis in mice.World journal of gastroenterology · 2014Article
- Large scale explorative oligonucleotide probe selection for thousands of genetic groups on a computing grid: application to phylogenetic probe design using a curated small subunit ribosomal RNA gene database.TheScientificWorldJournal · 2014Article
- Transcriptomics to study the effect of a Mediterranean-inspired diet on inflammation in Crohn's disease patients.Human genomics · 2013Article
- A DNA microarray for the versatile diagnosis of infectious diarrhea.APMIS : acta pathologica, microbiologica, et immunologica Scandinavica · 2013Article
- Article
- Flagellum density regulates Proteus mirabilis swarmer cell motility in viscous environments.Journal of bacteriology · 2013Article
- DNA microarrays for the diagnosis of infectious diseases.Medecine et maladies infectieuses · 2012Review
- Molecular analysis of meso- and thermophilic microbiota associated with anaerobic biowaste degradation.BMC microbiology · 2012Article
- Unbalance of intestinal microbiota in atopic children.BMC microbiology · 2012Article
- Application of phylogenetic microarrays to interrogation of human microbiota.FEMS microbiology ecology · 2012Review
- Detection of human papillomaviruses by polymerase chain reaction and ligation reaction on universal microarray.PloS one · 2012Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAffecting the core functional microbiome, peculiar high level taxonomic unbalances of the human intestinal microbiota have been recently associated with specific diseases, such as obesity, inflammatory bowel diseases, and intestinal inflammation.
resultsIn order to specifically monitor microbiota unbalances that impact human physiology, here we develop and validate an original DNA-microarray (HTF-Microbi.Array) for the high taxonomic level fingerprint of the human intestinal microbiota. Based on the Ligase Detection Reaction-Universal Array (LDR-UA) approach, the HTF-Microbi.Array enables specific detection and approximate relative quantification of 16S rRNAs from 30 phylogenetically related groups of the human intestinal microbiota. The HTF-Microbi.Array was used in a pilot study of the faecal microbiota of eight young adults. Cluster analysis revealed the good reproducibility of the high level taxonomic microbiota fingerprint obtained for each of the subject.
conclusionThe HTF-Microbi.Array is a fast and sensitive tool for the high taxonomic level fingerprint of the human intestinal microbiota in terms of presence/absence of the principal groups. Moreover, analysis of the relative fluorescence intensity for each probe pair of our LDR-UA platform can provide estimation of the relative abundance of the microbial target groups within each samples. Focusing the phylogenetic resolution at division, order and cluster levels, the HTF-Microbi.Array is blind with respect to the inter-individual variability at the species level.
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