ArticleReproductive biology and endocrinology : RB&E2010
Hormonal induction of spawning in 4 species of frogs by coinjection with a gonadotropin-releasing hormone agonist and a dopamine antagonist.
Article in Reproductive biology and endocrinology : RB&E, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 91 citations in OpenAlex.
- Exposure to Environmental Microbes Alters Responsiveness of Tadpole Gut Microbiome to Dietary Tannins.Integrative zoology · 2026Article
- Reception of conspecific cues alters testicular gene expression and improves fertility in boreal chorus frogs (Pseudacris maculata).Scientific reports · 2026Article
- Reproductive Modes of Amphibians and Reptiles: Impacts on Conservation and the Development of Assisted Reproductive Technologies.Advances in experimental medicine and biology · 2026Review
- Relationships between Pre- and Postcopulatory Sexually Selected Traits in Green Frogs (Integrative organismal biology (Oxford, England) · 2025Article
- Efficacy of salmon GnRHa, Ovaprim® and hCG for hormonal stimulation of spermiation in the Fowler's toad (Conservation physiology · 2024Article
- Gamete Collection, Artificial Fertilization and Captive-Rearing of Eggs in a Terrestrial-Breeding Anuran with Parental Care:Animals : an open access journal from MDPI · 2023Article
- Recovery and Characterization of Spermatozoa in a Neotropical, Terrestrial, Direct-Developing Riparian Frog (Animals : an open access journal from MDPI · 2023Article
- Application of Reproductive Technologies to the Critically Endangered Baw Baw Frog,Animals : an open access journal from MDPI · 2023Article
- The microbiome buffers tadpole hosts from heat stress: a hologenomic approach to understand host-microbe interactions under warming.The Journal of experimental biology · 2023Article
- Influence of oviposition-inducing hormone on spawning and mortality in the endangered Panamanian golden frog (Atelopus zeteki).BMC zoology · 2021Article
- Amphibian reproductive technologies: approaches and welfare considerations.Conservation physiology · 2021Article
- Ultrasound imaging improves hormone therapy strategies for induction of ovulation andConservation physiology · 2018Article
- Maternal body condition influences magnitude of anti-predator response in offspring.Proceedings. Biological sciences · 2014Article
- Efficient induction of spawning of northern leopard frogs (Lithobates pipiens) during and outside the natural breeding season.Reproductive biology and endocrinology : RB&E · 2013Article
- Optimisation of an oviposition protocol employing human chorionic and pregnant mare serum gonadotropins in the barred frog Mixophyes fasciolatus (Myobatrachidae).Reproductive biology and endocrinology : RB&E · 2012Article
- A comparison of human chorionic gonadotropin and luteinizing hormone releasing hormone on the induction of spermiation and amplexus in the American toad (Anaxyrus americanus).Reproductive biology and endocrinology : RB&E · 2012Article
- Hormonal induction of gamete release, and in-vitro fertilisation, in the critically endangered southern corroboree frog, Pseudophryne corroboree.Reproductive biology and endocrinology : RB&E · 2010Article
- Effects of luteinizing hormone-releasing hormone and arginine-vasotocin on the sperm-release response of Günther's Toadlet, Pseudophryne guentheri.Reproductive biology and endocrinology : RB&E · 2010Article
Corrections and comments
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Authors and funding
8 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIt is well known that many anurans do not reproduce easily in captivity. Some methods are based on administration of mammalian hormones such as human chorionic gonadotropin, which are not effective in many frogs. There is a need for simple, cost-effective alternative techniques to induce spawning.
methodsOur new method is based on the injection of a combination of a gonadotropin-releasing hormone (GnRH) agonist and a dopamine antagonist. We have named this formulation AMPHIPLEX, which is derived from the combination of the words amphibian and amplexus. This name refers to the specific reproductive behavior of frogs when the male mounts and clasps the female to induce ovulation and to fertilize the eggs as they are laid.
resultsWe describe the use of the method and demonstrate its applicability for captive breeding in 3 different anuran families. We tested several combinations of GnRH agonists with dopamine antagonists using Lithobates pipiens. The combination of des-Gly10, D-Ala6, Pro-LHRH (0.4 microrams/g body weight) and metoclopramide (10 micrograms/g BWt. MET) was most effective. It was used in-season, after short-term captivity and in frogs artificially hibernated under laboratory conditions. The AMPHIPLEX method was also effective in 3 Argentinian frogs, Ceratophrys ornata, Ceratophrys cranwelli and Odontophrynus americanus.
conclusionOur approach offers some advantages over other hormonally-based techniques. Both sexes are injected only once and at the same time, reducing handling stress. AMPHIPLEX is a new reproductive management tool for captive breeding in Anura.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.