ArticleGenome biology2010
Monovalent and unpoised status of most genes in undifferentiated cell-enriched Drosophila testis.
Article in Genome biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 74 citations in OpenAlex.
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- YY1 and CP2c in Unidirectional Spermatogenesis and Stemness.Development & reproduction · 2020Review
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- Transcriptional Dynamics of Grain Development in Barley (International journal of molecular sciences · 2019Article
- Epigenetic analyses of planarian stem cells demonstrate conservation of bivalent histone modifications in animal stem cells.Genome research · 2018Article
- Protecting and Diversifying the Germline.Genetics · 2018Review
- In situ functional dissection of RNA cis-regulatory elements by multiplex CRISPR-Cas9 genome engineering.Nature communications · 2017Article
- Polycomb Group Gene E(z) Is Required for Spermatogonial Dedifferentiation in Drosophila Adult Testis.Journal of molecular biology · 2017Article
- The Transcriptional Corepressor SIN3 Directly Regulates Genes Involved in Methionine Catabolism and Affects Histone Methylation, Linking Epigenetics and Metabolism.The Journal of biological chemistry · 2017Article
- Enhancer of polycomb coordinates multiple signaling pathways to promote both cyst and germline stem cell differentiation in the Drosophila adult testis.PLoS genetics · 2017Article
- The antenna transcriptome changes in mosquito Anopheles sinensis, pre- and post- blood meal.PloS one · 2017Article
- Integrative analysis with ChIP-seq advances the limits of transcript quantification from RNA-seq.Genome research · 2016Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundIncreasing evidence demonstrates that stem cells maintain their identities by a unique transcription network and chromatin structure. Opposing epigenetic modifications H3K27 me3 and H3K4 me3 have been proposed to label differentiation-associated genes in stem cells, progenitor and precursor cells. In addition, many differentiation-associated genes are maintained at a poised status by recruitment of the initiative RNA Polymerase II (Pol II) at their promoter regions, in preparation for lineage-specific expression upon differentiation. Previous studies have been performed using cultured mammalian embryonic stem cells. To a lesser extent, chromatin structure has been delineated in other model organisms, such as Drosophila, to open new avenues for genetic analyses.
resultsHere we use testes isolated from a Drosophila bag of marbles mutant strain, from which germ cells are in their undifferentiated status. We use these testes to study the endogenous chromatin structure of undifferentiated cells using ChIP-seq. We integrate the ChIP-seq with RNA-seq data, which measures the digital transcriptome. Our genome-wide analyses indicate that most differentiation-associated genes in undifferentiated cells lack an active chromatin mark and initiative Pol II; instead, they are associated with either the repressive H3K27 me3 mark or no detectable mark.
conclusionsOur results reveal that most of the differentiation-associated genes in undifferentiated-cell-enriched Drosophila testes are associated with monovalent but not bivalent modifications, a chromatin signature that is distinct from the data reported in mammalian stem or precursor cells, which may reflect cell type specificity, species specificity, or both.
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