ArticleJournal of the American Chemical Society2010
FRET-enabled optical modulation for high sensitivity fluorescence imaging.
Article in Journal of the American Chemical Society, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 32 citations in OpenAlex.
- Out-of-Phase Imaging after Optical Modulation (OPIOM) for Multiplexed Fluorescence Imaging Under Adverse Optical Conditions.Methods in molecular biology (Clifton, N.J.) · 2021Article
- A Pterin-FAM-TAMRA Tri-color Fluorescence Biosensor to Detect the Level of KRAS Point Mutation.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2020Article
- Time-Resolved Spectroscopy of Fluorescence Quenching in Optical Fibre-Based pH Sensors.Sensors (Basel, Switzerland) · 2020Article
- Developing and Comparing 2,6-Anthracene Derivatives: Optical, Electrochemical, Thermal, and Their Use in Organic Thin Film Transistors.Materials (Basel, Switzerland) · 2020Article
- Dark State-Modulated Fluorescence Correlation Spectroscopy for Quantitative Signal Recovery.The journal of physical chemistry letters · 2016Article
- Expanding discriminative dimensions for analysis and imaging.Chemical science · 2015Article
- Optically modulated fluorescence bioimaging: visualizing obscured fluorophores in high background.Accounts of chemical research · 2014Review
- Signal Discrimination Between Fluorescent Proteins in Live Cells by Long-wavelength Optical Modulation.The journal of physical chemistry letters · 2012Article
- Optical modulation and selective recovery of Cy5 fluorescence.Chemphyschem : a European journal of chemical physics and physical chemistry · 2012Article
- Optical imaging with dynamic contrast agents.Chemistry (Weinheim an der Bergstrasse, Germany) · 2011Article
- Plasmon-resonant nanoparticles and nanostars with magnetic cores: synthesis and magnetomotive imaging.ACS nano · 2010Article
- All-optical fluorescence image recovery using modulated Stimulated Emission Depletion.Chemical scienceArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Fluorescence resonance energy transfer is utilized to engineer donor photophysics for facile signal amplification and selective fluorescence recovery from high background. This is generalized such that many different fluorophores can be used in optical modulation schemes to drastically improve fluorescence imaging sensitivity. Dynamic, simultaneous, and direct excitation of the acceptor brightens and optically modulates higher energy donor emission. The externally imposed modulation waveform enables selective donor fluorescence extraction through demodulation. By incorporating an acceptor with significant, spectrally shifted, dark-state population, necessary excitation intensities are quite low and agree well with simulated enhancements. Enhancement versus modulation frequency directly yields dark-state lifetimes in a simple ensemble measurement. Using the long-lived Cy5 dark state in conjunction with Cy3 donors, we demonstrate image extraction from a large background to yield >>10-fold sensitivity improvements through synchronously amplified fluorescence image recovery (SAFIRe).
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.