ArticleMolecular & cellular proteomics : MCP2010
Integrated phosphoproteomics analysis of a signaling network governing nutrient response and peroxisome induction.
Article in Molecular & cellular proteomics : MCP, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 37 citations in OpenAlex.
- A Labrador PeptideAtlas and DIA spectral assay library - resources for proteomics research in dogs.Scientific data · 2026Article
- Uip4p modulates nuclear pore complex function inNucleus (Austin, Tex.) · 2022Article
- Pex30 undergoes phosphorylation and regulates peroxisome number in Saccharomyces cerevisiae.Molecular genetics and genomics : MGG · 2022Article
- The Pivotal Role of Protein Phosphorylation in the Control of Yeast Central Metabolism.G3 (Bethesda, Md.) · 2017Article
- Bioactive Nutrients and Nutrigenomics in Age-Related Diseases.Molecules (Basel, Switzerland) · 2017Review
- Article
- Signaling dynamics and peroxisomes.Current opinion in cell biology · 2015Review
- Phosphoproteome Analysis Links Protein Phosphorylation to Cellular Remodeling and Metabolic Adaptation during Magnaporthe oryzae Appressorium Development.Journal of proteome research · 2015Article
- Phosphoproteome dynamics of Saccharomyces cerevisiae under heat shock and cold stress.Molecular systems biology · 2015Article
- Affinity proteomics to study endogenous protein complexes: pointers, pitfalls, preferences and perspectives.BioTechniques · 2015Article
- Cross-talk phosphorylations by protein kinase C and Pho85p-Pho80p protein kinase regulate Pah1p phosphatidate phosphatase abundance in Saccharomyces cerevisiae.The Journal of biological chemistry · 2014Article
- The yeast Sks1p kinase signaling network regulates pseudohyphal growth and glucose response.PLoS genetics · 2014Article
- Peroxisomes take shape.Nature reviews. Molecular cell biology · 2013Review
- Combining results of multiple search engines in proteomics.Molecular & cellular proteomics : MCP · 2013Review
- Reverse engineering systems models of regulation: discovery, prediction and mechanisms.Current opinion in biotechnology · 2012Review
- The peroxisome: an update on mysteries.Histochemistry and cell biology · 2012Review
- Phosphoproteomic analysis: an emerging role in deciphering cellular signaling in human embryonic stem cells and their differentiated derivatives.Stem cell reviews and reports · 2012Review
- The yeast nuclear pore complex and transport through it.Genetics · 2012Article
- The yeast magmas ortholog pam16 has an essential function in fermentative growth that involves sphingolipid metabolism.PloS one · 2012Article
- Defining the plant peroxisomal proteome: from Arabidopsis to rice.Frontiers in plant science · 2011Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
Abstract
Phosphorylation of proteins is a key posttranslational modification in cellular signaling, regulating many aspects of cellular responses. We used a quantitative, integrated, phosphoproteomics approach to characterize the cellular responses of the yeast Saccharomyces cerevisiae to the fatty acid oleic acid, a molecule with broad human health implications and a potent inducer of peroxisomes. A combination of cryolysis and urea solubilization was used to minimize the opportunity for reorientation of the phosphoproteome, and hydrophilic interaction liquid chromatography and IMAC chemistries were used to fractionate and enrich for phosphopeptides. Using these approaches, numerous phosphorylated peptides specific to oleate-induced and glucose-repressed conditions were identified and mapped to known signaling pathways. These include several transcription factors, two of which, Pip2p and Cst6p, must be phosphorylated for the normal transcriptional response of fatty acid-responsive loci encoding peroxisomal proteins. The phosphoproteome data were integrated with results from genome-wide assays studying the effects of signaling molecule deletions and known protein-protein interactions to generate a putative fatty acid-responsive signaling network. In this network, the most highly connected nodes are those with the largest effects on cellular responses to oleic acid. These properties are consistent with a scale-free topology, demonstrating that scale-free properties are conserved in condition-specific networks.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.